REPIN1
Replication initiator 1 is a protein that in humans is encoded by the REPIN1 gene.[5][6]
Model organisms
Model organisms have been used in the study of REPIN1 function. A conditional knockout mouse line called Repin1tm1a(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute.[7] Male and female animals underwent a standardized phenotypic screen[8] to determine the effects of deletion.[9][10][11][12] Additional screens performed: - In-depth immunological phenotyping[13]
| Characteristic | Phenotype |
|---|---|
| All data available at.[8][13] | |
| Peripheral blood leukocytes 6 Weeks | Normal |
| Insulin | Normal |
| Haematology 6 Weeks | Normal |
| Homozygous viability at P14 | Normal |
| Homozygous Fertility | Normal |
| Body weight | Normal |
| Neurological assessment | Normal |
| Grip strength | Normal |
| Dysmorphology | Normal |
| Indirect calorimetry | Abnormal |
| Glucose tolerance test | Normal |
| Auditory brainstem response | Normal |
| DEXA | Normal |
| Radiography | Normal |
| Eye morphology | Normal |
| Clinical chemistry | Normal |
| Haematology 16 Weeks | Normal |
| Peripheral blood leukocytes 16 Weeks | Normal |
| Heart weight | Normal |
| Salmonella infection | Normal |
| Cytotoxic T Cell Function | Normal |
| Spleen Immunophenotyping | Normal |
| Mesenteric Lymph Node Immunophenotyping | Normal |
| Bone Marrow Immunophenotyping | Normal |
| Epidermal Immune Composition | Normal |
References
- GRCh38: Ensembl release 89: ENSG00000214022 - Ensembl, May 2017
- GRCm38: Ensembl release 89: ENSMUSG00000052751 - Ensembl, May 2017
- "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- Houchens CR, Montigny W, Zeltser L, Dailey L, Gilbert JM, Heintz NH (Jan 2000). "The dhfr oribeta-binding protein RIP60 contains 15 zinc fingers: DNA binding and looping by the central three fingers and an associated proline-rich region". Nucleic Acids Research. 28 (2): 570–81. doi:10.1093/nar/28.2.570. PMC 102514. PMID 10606657.
- "Entrez Gene: REPIN1 replication initiator 1".
- Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
- "International Mouse Phenotyping Consortium".
- Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
- White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
- "Infection and Immunity Immunophenotyping (3i) Consortium".
Further reading
- Dailey L, Caddle MS, Heintz N, Heintz NH (Dec 1990). "Purification of RIP60 and RIP100, mammalian proteins with origin-specific DNA-binding and ATP-dependent DNA helicase activities". Molecular and Cellular Biology. 10 (12): 6225–35. doi:10.1128/mcb.10.12.6225. PMC 362897. PMID 2174103.
- Caddle MS, Dailey L, Heintz NH (Dec 1990). "RIP60, a mammalian origin-binding protein, enhances DNA bending near the dihydrofolate reductase origin of replication". Molecular and Cellular Biology. 10 (12): 6236–43. doi:10.1128/mcb.10.12.6236. PMC 362898. PMID 2247056.
- Mastrangelo IA, Held PG, Dailey L, Wall JS, Hough PV, Heintz N, Heintz NH (Aug 1993). "RIP60 dimers and multiples of dimers assemble link structures at an origin of bidirectional replication in the dihydrofolate reductase amplicon of Chinese hamster ovary cells". Journal of Molecular Biology. 232 (3): 766–78. doi:10.1006/jmbi.1993.1430. PMID 8355269.
- Montigny WJ, Houchens CR, Illenye S, Gilbert J, Coonrod E, Chang YC, Heintz NH (May 2001). "Condensation by DNA looping facilitates transfer of large DNA molecules into mammalian cells". Nucleic Acids Research. 29 (9): 1982–8. doi:10.1093/nar/29.9.1982. PMC 37261. PMID 11328883.
- Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, Li Y, Xu C, Fang R, Guegler K, Rao MS, Mandalam R, Lebkowski J, Stanton LW (Jun 2004). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nature Biotechnology. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.
- Kim MY, Jeong BC, Lee JH, Kee HJ, Kook H, Kim NS, Kim YH, Kim JK, Ahn KY, Kim KK (Aug 2006). "A repressor complex, AP4 transcription factor and geminin, negatively regulates expression of target genes in nonneuronal cells". Proceedings of the National Academy of Sciences of the United States of America. 103 (35): 13074–9. doi:10.1073/pnas.0601915103. PMC 1551900. PMID 16924111.
External links
- REPIN1+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH)
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