TRPV6

TRPV6 is a membrane calcium channel protein which is particularly involved in the first step in calcium absorption in the intestine.

TRPV6
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesTRPV6, ABP/ZF, CAT1, CATL, ECAC2, HSA277909, LP6728, ZFAB, transient receptor potential cation channel subfamily V member 6, HRPTTN
External IDsOMIM: 606680 MGI: 1927259 HomoloGene: 56812 GeneCards: TRPV6
Gene location (Human)
Chr.Chromosome 7 (human)[1]
Band7q34Start142,871,208 bp[1]
End142,885,745 bp[1]
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez

55503

64177

Ensembl

ENSG00000276971
ENSG00000165125

ENSMUSG00000029868

UniProt

Q9H1D0

Q91WD2

RefSeq (mRNA)

NM_018646
NM_014274

NM_022413

RefSeq (protein)

NP_061116

NP_071858

Location (UCSC)Chr 7: 142.87 – 142.89 MbChr 6: 41.62 – 41.64 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Nomenclature

When first discovered it was named CAT1,[5] or ECaC2.[6] The name TRPV6 was confirmed in 2005.[7]

TRPV6 is a member of the transient receptor potential (TRP) family of membrane proteins. Unlike most TRP channels, TRPV6 is selective for Ca2+ ions, a property shared with its close homologue, TRPV5, which is mainly expressed in the kidney and plays a role in renal Ca2+ reabsorption.[8]

Expression

TRPV6 expression has been described in the intestine in several species, including humans.[9] Both TRPV5 and TRPV6 are expressed in kidney and intestinal epithelial cells, but TRPV5 is mainly expressed in kidney epithelial cells, whereas TRPV6 is mainly expressed in the intestine.[10] TRPV6 facilitates calcium transport in intestinal cells.[10]

The TRPV6 protein is located in the apical brush-border membrane of the intestinal enterocyte where it regulates calcium entry into the cell. It is most abundant in the proximal small intestine (duodenum and jejunum), along with the other calcium transport proteins, calbindin and the calcium-pumping ATPase, PMCA1. The TRPV6 calcium transporter also found in the human placenta, pancreas and prostate gland and in some species in the kidney, where the related channel TRPV5 is strongly expressed.

Regulation of expression

Vitamin D

Expression of TRPV6 is vitamin D dependent in mice and humans. Its expression was greatly reduced in animals that do not express the vitamin D receptor.[11]

Vitamin D treatment of human colon cancer cells, Caco-2, increased expression of TRPV6 transcripts, and also stimulated the transport of calcium, probably through increased TRPV6 expression.[12] In human duodenal explants, TRPV6 transcript expression was increased 3-fold after 6h incubation with the active form of vitamin D, 1,25-dihydroxycholecalciferol.[13]

See also

References
  1. ENSG00000165125 GRCh38: Ensembl release 89: ENSG00000276971, ENSG00000165125 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000029868 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Peng JB, Chen XZ, Berger UV, Vassilev PM, Tsukaguchi H, Brown EM, Hediger MA (August 1999). "Molecular cloning and characterization of a channel-like transporter mediating intestinal calcium absorption". J. Biol. Chem. 274 (32): 22739–46. doi:10.1074/jbc.274.32.22739. PMID 10428857.
  6. Weber K, Erben RG, Rump A, Adamski J (December 2001). "Gene structure and regulation of the murine epithelial calcium channels ECaC1 and 2". Biochem. Biophys. Res. Commun. 289 (5): 1287–94. doi:10.1006/bbrc.2001.6121. PMID 11741335.
  7. Clapham DE, Julius D, Montell C, Schultz G (December 2005). "International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels". Pharmacol. Rev. 57 (4): 427–50. doi:10.1124/pr.57.4.6. PMID 16382100.
  8. Peng JB, Brown EM, Hediger MA (September 2003). "Epithelial Ca2+ entry channels: transcellular Ca2+ transport and beyond". J. Physiol. 551 (Pt 3): 729–40. doi:10.1113/jphysiol.2003.043349. PMC 2343303. PMID 12869611.
  9. Barley NF, Howard A, O'Callaghan D, Legon S, Walters JR (February 2001). "Epithelial calcium transporter expression in human duodenum". Am. J. Physiol. Gastrointest. Liver Physiol. 280 (2): G285–90. doi:10.1152/ajpgi.2001.280.2.G285. PMID 11208552.
  10. van Goor MK, Hoenderop JG, van der Wijst J (2017). "TRP channels in calcium homeostasis: from hormonal control to structure-function relationship of TRPV5 and TRPV6". Biochimica et Biophysica Acta. 1864 (6): 883–893. doi:10.1016/j.bbamcr.2016.11.027. PMID 27913205.
  11. Van Cromphaut SJ, Dewerchin M, Hoenderop JG, Stockmans I, Van Herck E, Kato S, Bindels RJ, Collen D, Carmeliet P, Bouillon R, Carmeliet G (November 2001). "Duodenal calcium absorption in vitamin D receptor-knockout mice: functional and molecular aspects". Proc. Natl. Acad. Sci. U.S.A. 98 (23): 13324–9. doi:10.1073/pnas.231474698. PMC 60869. PMID 11687634.
  12. Taparia S, Fleet JC, Peng JB, Wang XD, Wood RJ (June 2006). "1,25-Dihydroxyvitamin D and 25-hydroxyvitamin D--mediated regulation of TRPV6 (a putative epithelial calcium channel) mRNA expression in Caco-2 cells". Eur J Nutr. 45 (4): 196–204. doi:10.1007/s00394-005-0586-3. PMID 16362534.
  13. Balesaria S, Sangha S, Walters JR (December 2009). "Human duodenum responses to vitamin D metabolites of TRPV6 and other genes involved in calcium absorption". Am. J. Physiol. Gastrointest. Liver Physiol. 297 (6): G1193–7. doi:10.1152/ajpgi.00237.2009. PMC 2850091. PMID 19779013.

Further reading
  • Heiner I, Eisfeld J, Lückhoff A (2004). "Role and regulation of TRP channels in neutrophil granulocytes". Cell Calcium. 33 (5–6): 533–40. doi:10.1016/S0143-4160(03)00058-7. PMID 12765698.
  • Clapham DE, Julius D, Montell C, Schultz G (2006). "International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels". Pharmacol. Rev. 57 (4): 427–50. doi:10.1124/pr.57.4.6. PMID 16382100.
  • Wissenbach U, Niemeyer BA (2007). "TRPV6". Handb Exp Pharmacol. Handbook of Experimental Pharmacology. 179 (179): 221–34. doi:10.1007/978-3-540-34891-7_13. ISBN 978-3-540-34889-4. PMID 17217060.
  • Schoeber JP, Hoenderop JG, Bindels RJ (2007). "Concerted action of associated proteins in the regulation of TRPV5 and TRPV6". Biochem. Soc. Trans. 35 (Pt 1): 115–9. doi:10.1042/BST0350115. PMID 17233615.


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