NT5E

NT5E
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesNT5E, CALJA, CD73, E5NT, NT, NT5, NTE, eN, eNT, 5'-nucleotidase ecto
External IDsMGI: 99782 HomoloGene: 1895 GeneCards: NT5E
Gene location (Human)
Chr.Chromosome 6 (human)[1]
Band6q14.3Start85,449,584 bp[1]
End85,495,791 bp[1]
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez

4907

23959

Ensembl

ENSG00000135318

ENSMUSG00000032420

UniProt

P21589

Q61503

RefSeq (mRNA)

NM_002526
NM_001204813

NM_011851

RefSeq (protein)

NP_001191742
NP_002517

NP_035981

Location (UCSC)Chr 6: 85.45 – 85.5 MbChr 9: 88.33 – 88.37 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that in humans is encoded by the NT5E gene.[5] CD73 commonly serves to convert AMP to adenosine.

Function

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[6] Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.[7][8][9]

See also

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000135318 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000032420 - Ensembl, May 2017
  3. ↑ "Human PubMed Reference:".
  4. ↑ "Mouse PubMed Reference:".
  5. ↑ Misumi Y, Ogata S, Ohkubo K, Hirose S, Ikehara Y (August 1990). "Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form". European Journal of Biochemistry. 191 (3): 563–9. doi:10.1111/j.1432-1033.1990.tb19158.x. PMID 2129526.
  6. ↑ "Entrez Gene: NT5E 5'-nucleotidase, ecto (CD73)".
  7. ↑ St Hilaire C, Ziegler SG, Markello TC, Brusco A, Groden C, Gill F, Carlson-Donohoe H, Lederman RJ, Chen MY, Yang D, Siegenthaler MP, Arduino C, Mancini C, Freudenthal B, Stanescu HC, Zdebik AA, Chaganti RK, Nussbaum RL, Kleta R, Gahl WA, Boehm M (February 2011). "NT5E mutations and arterial calcifications". The New England Journal of Medicine. 364 (5): 432–42. doi:10.1056/NEJMoa0912923. PMC 3049958. PMID 21288095.
  8. ↑ Sharp J (March 1954). "Heredo-familial vascular and articular calcification". Annals of the Rheumatic Diseases. 13 (1): 15–27. doi:10.1136/ard.13.1.15. PMC 1030367. PMID 13149051.
  9. ↑ Online Mendelian Inheritance in Man (OMIM) 211800

Further reading

  • Resta R, Thompson LF (February 1997). "T cell signalling through CD73". Cellular Signalling. 9 (2): 131–9. doi:10.1016/S0898-6568(96)00132-5. PMID 9113412.
  • Kirchhoff C, Hale G (March 1996). "Cell-to-cell transfer of glycosylphosphatidylinositol-anchored membrane proteins during sperm maturation". Molecular Human Reproduction. 2 (3): 177–84. doi:10.1093/molehr/2.3.177. PMID 9238677.
  • Resta R, Yamashita Y, Thompson LF (February 1998). "Ecto-enzyme and signaling functions of lymphocyte CD73". Immunological Reviews. 161: 95–109. doi:10.1111/j.1600-065X.1998.tb01574.x. PMID 9553767.
  • Rosi F, Carlucci F, Marinello E, Tabucchi A (March 2002). "Ecto-5'-nucleotidase in B-cell chronic lymphocytic leukemia". Biomedicine & Pharmacotherapy. 56 (2): 100–4. doi:10.1016/S0753-3322(01)00072-5. PMID 12000134.
  • Babiychuk EB, Draeger A (June 2006). "Regulation of ecto-5'-nucleotidase activity via Ca2+-dependent, annexin 2-mediated membrane rearrangement?". Biochemical Society Transactions. 34 (Pt 3): 374–6. doi:10.1042/BST0340374. PMID 16709165.
  • Stefanovic V, Mandel P, Rosenberg A (July 1976). "Ecto-5'-nucleotidase of intact cultured C6 rat glioma cells". The Journal of Biological Chemistry. 251 (13): 3900–5. PMID 819433.
  • Thomson LF, Ruedi JM, Glass A, Moldenhauer G, Moller P, Low MG, Klemens MR, Massaia M, Lucas AH (January 1990). "Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73)". Tissue Antigens. 35 (1): 9–19. doi:10.1111/j.1399-0039.1990.tb01750.x. PMID 2137649.
  • Klemens MR, Sherman WR, Holmberg NJ, Ruedi JM, Low MG, Thompson LF (November 1990). "Characterization of soluble vs membrane-bound human placental 5'-nucleotidase". Biochemical and Biophysical Research Communications. 172 (3): 1371–7. doi:10.1016/0006-291X(90)91601-N. PMID 2173922.
  • Boyle JM, Hey Y, Guerts van Kessel A, Fox M (December 1988). "Assignment of ecto-5'-nucleotidase to human chromosome 6". Human Genetics. 81 (1): 88–92. doi:10.1007/BF00283737. PMID 2848759.
  • Vlahović P, Stefanović V (1995). "Effect of dopamine on ecto-5'-nucleotidase expression in human glomerular mesangial cells". Archives Internationales De Physiologie, De Biochimie Et De Biophysique. 102 (3): 171–3. PMID 8000038.
  • Hansen KR, Resta R, Webb CF, Thompson LF (December 1995). "Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene". Gene. 167 (1–2): 307–12. doi:10.1016/0378-1119(95)00574-9. PMID 8566797.
  • Airas L, Jalkanen S (September 1996). "CD73 mediates adhesion of B cells to follicular dendritic cells". Blood. 88 (5): 1755–64. PMID 8781432.
  • Airas L, Niemelä J, Salmi M, Puurunen T, Smith DJ, Jalkanen S (January 1997). "Differential regulation and function of CD73, a glycosyl-phosphatidylinositol-linked 70-kD adhesion molecule, on lymphocytes and endothelial cells". The Journal of Cell Biology. 136 (2): 421–31. doi:10.1083/jcb.136.2.421. PMC 2134816. PMID 9015312.
  • Strohmeier GR, Lencer WI, Patapoff TW, Thompson LF, Carlson SL, Moe SJ, Carnes DK, Mrsny RJ, Madara JL (June 1997). "Surface expression, polarization, and functional significance of CD73 in human intestinal epithelia". The Journal of Clinical Investigation. 99 (11): 2588–601. doi:10.1172/JCI119447. PMC 508104. PMID 9169488.
  • Aumüller G, Renneberg H, Schiemann PJ, Wilhelm B, Seitz J, Konrad L, Wennemuth G (1998). "The role of apocrine released proteins in the post-testicular regulation of human sperm function". Advances in Experimental Medicine and Biology. 424: 193–219. doi:10.1007/978-1-4615-5913-9_39. PMID 9361795.
  • Rosi F, Agostinho AB, Carlucci F, Zanoni L, Porcelli B, Marinello E, Galieni P, Tabucchi A (1998). "Behaviour of human lymphocytic isoenzymes of 5'-nucleotidase". Life Sciences. 62 (25): 2257–66. doi:10.1016/S0024-3205(98)00206-9. PMID 9651114.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.


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