Nuclear-inclusion-a endopeptidase

Nuclear-inclusion-a endopeptidase
Identifiers
EC number 3.4.22.44
CAS number 139946-51-3
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Nuclear-inclusion-a endopeptidase (EC 3.4.22.44, potyvirus NIa protease) is a protease enzyme found in potyviruses.[1][2][3][4] This enzyme catalyses the following chemical reaction:

Hydrolyses glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus.
e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln \ (Ser or Gly) for the enzyme from tobacco etch virus.

The enzyme is used encoded in vectors for the artificial expression of recombinant fusion proteins (see TEV protease).

References

  1. ↑ Fellers, J.P.; Collins, G.B.; Hunt, A.G. (1998). "The NIa-proteinase of different plant potyviruses provides specific resistance to viral infection". Crop Sci. 38: 1309–1319. doi:10.2135/cropsci1998.0011183x003800050030x.
  2. ↑ Kim, D.-H.; Choi, K.Y. (1998). "Potyvirus NIa protease". In Barrett, A.J.; Rawlings, N.D.; Woessner, J.F. Handbook of Proteolytic Enzymes. London: Academic Press. pp. 721–723.
  3. ↑ Takahashi, T.; Nakanishi, M.; Yao, Y.; Uyeda, I.; Serizawa, N. (1998). "Direct formation of human interleukin-11 by cis-acting system of plant virus protease in Escherichia coli". Biosci. Biotechnol. Biochem. 62: 953–958. doi:10.1271/bbb.62.953. PMID 9648226.
  4. ↑ Kim, D.H.; Hwang, D.C.; Kang, B.H.; Lew, J.; Han, J.S.; Song, B.O.D.; Choi, K.Y. (1996). "Effects of internal cleavages and mutations in the C-terminal region of NIa protease of turnip mosaic potyvirus on the catalytic activity". Virology. 226: 183–190. doi:10.1006/viro.1996.0645. PMID 8955037.
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