Cyanophycinase

Cyanophycinase
Identifiers
EC number 3.4.15.6
CAS number 131554-16-0
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Cyanophycinase (EC 3.4.15.6, cyanophycin degrading enzyme, beta-Asp-Arg hydrolysing enzyme, CGPase, CphB, CphE, cyanophycin granule polypeptidase, extracellular CGPase) is an enzyme.[1][2][3] It catalyses the following chemical reaction

[L-Asp(4-L-Arg)]n + H2O [L-Asp(4-L-Arg)]n-1 + L-Asp(4-L-Arg)

The enzyme is highly specific for the branched polypeptide cyanophycin.

References

  1. ↑ Obst M, Krug A, Luftmann H, Steinbüchel A (July 2005). "Degradation of cyanophycin by Sedimentibacter hongkongensis strain KI and Citrobacter amalonaticus strain G Isolated from an anaerobic bacterial consortium". Applied and Environmental Microbiology. 71 (7): 3642–52. doi:10.1128/aem.71.7.3642-3652.2005. PMC 1169039. PMID 16000772.
  2. ↑ Obst M, Oppermann-Sanio FB, Luftmann H, Steinbüchel A (July 2002). "Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI. The cphE gene from P. anguilliseptica BI encodes a cyanophycinhydrolyzing enzyme". The Journal of Biological Chemistry. 277 (28): 25096–105. doi:10.1074/jbc.m112267200. PMID 11986309.
  3. ↑ Richter R, Hejazi M, Kraft R, Ziegler K, Lockau W (July 1999). "Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartic acid (cyanophycin): molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme". European Journal of Biochemistry. 263 (1): 163–9. doi:10.1046/j.1432-1327.1999.00479.x. PMID 10429200.
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