Spencer Smythe

• A similar character named Henry Smythe appeared in the 1967 Spider-Man animated series, voiced by Henry Ramer. He created the Spider-Slayers as seen in "Captured by J. Jonah Jameson" where he used a Spider-Slayer to capture Spider-Man. When Spider-Man fools the Spider-Slayer with a dummy, J. Jonah Jameson gets angered at Spider-Man's trick as Henry takes the Spider-Slayer away to do some adjustments.
  

  Spencer Smythe as seen in Spider-Man: The Animated Series.
• Spencer Smythe appeared in Spider-Man: The Animated Series, voiced by Edward Mulhare. This version was the Spider-Slayers' original creator. He was hired by Norman Osborn to create the Black Widow while Osborn would build a hoverchair for Alistair Smythe. At Oscorp, Spencer's Black Widow instead captures Flash Thompson (in a Spider-Man costume) and Spider-Man attempts a rescue which leads to the plant's massive fire. When Osborn refuses to build the promised hoverchair for his son if Spider-Man isn't destroyed, Spencer chooses to stay behind and finish Spider-Man off while Osborn gets Alistair to safety. After the Black Widow is destroyed by Spider-Man, Oscorp explodes and Spencer is believed dead. However, it's later revealed that Spencer had survived and was put in cryogenic suspension by the Kingpin in order to maintain Alistair's loyalty. His son (as the biomechanical Ultimate Spider-Slayer) eventually discovered Spencer's cryonically preserved body with Spider-Man's help. Afterwards, Alistair continued working for various resourceful individuals for his father's revival.
• Spencer Smythe appears in the 2010s Spider-Man animated series, voiced by Benjamin Diskin. This version is Peter Parker and Harry Osborn's strict science teacher at Midtown High. In the episode "Horizon High: Part 1", he is responsible for sabotaging Harry's experiment, resulting in Harry's suspension from Horizon High pending an investigation. Spencer is also allied with the Vulture as the two have personal issues with Max Modell and created the Spider-Slayer robot which stole Harry's work from Horizon High. It is implied in the episode "Osborn Academy" that he is partnered with Norman Osborn as Harry's equipment is shown at Oscorp after Osborn's appropriation. In the episode "Ultimate Spider-Man", Spencer controls his Spider-Slayer to attack Oscorp as a diversion so that he can steal the briefcase with Raymond Warren's spider experiments only to bump into Miles Morales and lose the Electrolis Arachnatis specimen. Spencer later uses a mecha Spider-Slayer personally and competes against his own son to catch the Ultimate Spider-Man. After ejecting from his Spider-Slayer upon defeat, Spencer crashes into the bullies that picked on Miles where he is then webbed up by Spider-Man and handed over to the police. While being walked to his prison cell, Spencer sees Raymond in a cell and asks how Raymond got back in prison; Raymond (actually an explosive clone in disguise) told him of having never leaving prison. To Spencer's further confusion, Warren is unfamiliar with him.

A viral marketing campaign for The Amazing Spider-Man 2 shows a mention of Spencer Smythe in a Daily Bugle article as Oscorp's head of the engineering division and that he is working on significant advancements in robotics. An article published at a later date revealed that he was fired from his position as department head and replaced by his son Alistair Smythe.[11][12]

• Spencer Smythe is featured in the PlayStation 2 and PSP version of Spider-Man: Web of Shadows. He calls Spider-Man at specific points of the game and is shown to be in alliance with A.I.M. whom he hires to capture J. Jonah Jameson in his plot to clone and gain control of the Daily Bugle, as well as discredit both Jameson and Spider-Man. Spencer is also in alliance with the Jackal to infiltrate the S.H.I.E.L.D. Helicarrier as a double agent to do research on the symbiotes invading Manhattan, which Spencer seeks to learn how to control and then use to take over the world. However, Jackal betrays Spencer and plans to obtain Spider-Man's symbiotic suit for his own purposes. Spencer later sends several A.I.M. agents to steal the Sonic Emitter meant to destroy the symbiotes from the Sonic top of Fisk Tower, but Jackal steals it instead whilist Spider-Man is busy fighting the A.I.M. agents. Spencer then calls Spider-Man and reveals that he had nothing to do with Jackal stealing the Sonic Emitter, claiming that whatever his plan is, it's too late to stop the symbiote invasion, before unleashing the mind-controlled Black Cat to kill Spider-Man.
• Spencer Smythe appears in Marvel Heroes.
• Spencer Smythe appears as the primary antagonist of The Amazing Spider-Man on Nintendo 3DS. He utilizes multiple heavily reimagined Spider-Slayers and a slew of robotic forces in an attempt to stop the uneasy alliance of Peter Parker and Curt Connors.