Spider-Man (2017 TV series)

Gifted teenager Peter Parker is given spider-like powers when he is bitten by a radioactive spider during a school field trip to Oscorp. After the death of his Uncle Ben by a burglar that he hesitated to stop at a wrestling arena, Parker comes to his own terms of being the masked superhero Spider-Man. Some time later, Peter begins learning how to balance his crime-fighting career with his new enrollment at Horizon High, a school for students with genius-level intellect run by headmaster Max Modell. Joining Peter in his ordeals against Jackal, Doctor Octopus, Vulture, Rhino, the Smythes (Spencer and Alistair), and numerous other villains are his classmates Gwen Stacy, Miles Morales, and Anya Corazon, as well as his best friend Harry Osborn.[5]

Over the course of the school year, Peter merges with a parasitic alien dubbed the V-252 which he later removes and trains Miles on how to use his own Spider Powers as an "Ultimate Spider-Man" after he ends up bitten by a genetically-modified spider as well. Peter and his friends eventually discover that Jackal (who is actually Gwen's uncle Raymond Warren) created the spiders that gave them their Spider Powers for the purpose of turning everyone in New York into an army of spider-like monsters dubbed "Man-Spiders", but Raymond's scheme is thwarted by the teamwork of Peter, Miles, Anya and Harry. During this period, Gwen and Anya develop Spider Powers of their own with the former assuming the "Spider-Gwen" alias.

With Osborn Academy destroyed at the climax of the "Spider-Island" crisis, Harry dons the Hobgoblin technology and discovers that his father Norman Osborn orchestrated his expulsion from Horizon High at the school year's beginning as part of his vision of making his son execute Spider-Man and be New York's definite hero. Ultimately, Harry chooses to side with Peter instead of his father, and Norman apparently dies in an explosion. As summer vacation begins, Harry takes over his father's business and discovers a hidden chamber filled with Norman's Goblin technology while Max creates a monument of Spider-Man at Horizon High as the school's unofficial mascot.

Peter navigates through both his sophomore year and his career at the Daily Bugle while finding that he must once again contend with Doctor Octopus as well as the V-252 Symbiote, which has now assumed the name of "Venom" after it merges itself with his jealous co-worker Eddie Brock. While Peter uncovers a bounty that has been seemingly placed on him by Chameleon, Anya adopts the "Spider-Girl" alias when her Spider Powers from the "Spider-Island" incident resurface and forms a "Spider Team" with Miles and Gwen. Doctor Octopus is revealed to be the true mastermind behind the bounty and successfully manages to swap bodies with Spider-Man, becoming the Superior Spider-Man. Around the same time, Gwen acquires old and new Spider Powers thanks to the Blood Gem relic and begins working alongside Miles and Anya under the "Ghost-Spider" alias.[6]

After being swayed by Peter's memories of Uncle Ben into becoming a hero, Octavius begins working on methods to become more efficient in crime-fighting than Peter ever was, but his ruthlessness prompts Miles to have the Avengers investigate on his behalf. Meanwhile, Peter's consciousness escapes the Living Brain via the Neuro Cortex and downloads himself into Octavius' discarded tentacles after encountering memories of Octavius' past in the process. Octavius (as Peter) re-enrolls at Midtown High and befriends the assistant chemistry teacher Anna Maria Marconi. Discovering how wrong he was about himself and Spider-Man during a fight against a genetically-altered Venom, Octavius reunites Peter's consciousness and body before his tentacles degrade the former into nothing. After saving Anna Maria from Venom, Spider-Man honors Octavius' heroism by placing his consciousness back in his inactive body. Soon after becoming his former self once more, Spider-Man takes it upon himself to rescue the Avengers from Regent, who has been replicating the abilities of the various super villains incarcerated at the prison known as the Cellar to imprison the Avengers in another dimension. Octavius recovers from his coma in time for him and Anna Maria to help Spider-Man free Cloak and Dagger so they can rescue the missing Avengers and defeat Regent.

After Spider-Man stops the rampaging speedster Slyde with help from Octavius (now going by Doctor Octopus once more), they discover the existence of a recently formed cult-like organization who are calling themselves the Goblin Nation, armed with the same kind of Goblin technology used by Norman. While investigating Norman's hidden Goblin tech chamber, Spider-Man and Hobgoblin discover the Goblin Nation's leader the Goblin King is none other than Adrian Toomes. Working together with the rest of the Spider Team, they are able to defeat the Goblin Nation's various clans (consisting of Electro's Electro Goblins, Silvermane's Cyber Goblins, and Crossbones' War Goblins). When Toomes takes control of a powerful Goblin Mech in a bid to destroy the city, Octavius stops him by disabling the suit with the Neuro Cortex but fades away into light particles in the process. At Octavius's funeral, Peter reconciles with Gwen, Anya, and Max for what happened when Octavius was Superior Spider-Man. To his surprise, Peter is re-enrolled at Horizon High thanks to a recommendation letter given to Max by Octavius prior to the latter's death.

Two weeks after Otto Octavius' death, Spider-Man finds himself dealing with a machine called the Technovore rampaging through Horizon High. He ends up using a synthetic copy of Venom created by Max to stop it, while Max also admits to Peter that he has been aware of his true identity since the downfall of the Goblin Nation. Peter and Max soon discover that the Technovore's rampage was orchestrated by the school's new teacher Curt Connors, as part of a mission to investigate Max's "corrupt operations" on the behalf of unknown benefactors. But during his investigation, Dr. Connors accidentally releases the original Venom, who revitalizes himself using his synthetic copy and escape to Duncan Valley. Recovering an energy seed from the meteor that brought him to Earth, Venom uses it to shoot out a beam-like beacon powerful enough to travel through space to his home planet before being ultimately killed by Spider-Man using electricity. A massive swarm of Klyntar to begin making their way to Earth for an invasion, capturing and possessing Captain Marvel, Iron Man, Captain America, Thor, and Star-Lord in the process. Groot is able to arrive on Earth ahead of the Klyntar to warn Spider-Man about the impending invasion, having helped him with a prior Venom-related incident. Peter and Miles are able to decode Groot's message after they, Ironheart, Totally Awesome Hulk, and Doctor Strange rescue Groot from the clutches of A.I.M. and Baron Mordo.

As the first wave of Klyntar make planetfall, they use the possessed Avengers as their advance team to possess the heroes of Earth one-by-one so the rest of the swarm can quickly do the same to the citizens of New York City, with only a few survivors including Spider-Man, Max, and Aunt May. Despite the growing threat caused by the invasion pushing him to his breaking point, Spider-Man makes an alliance with retired hero Marc Spector to rescue Max, who has captured the Venomized Groot so he can create an antidote to the Klyntar possession. With the help of Marc (who has renounced his retirement as Moon Knight), May, and the other survivors, Spider-Man and Max succeed in creating a synthetic Klyntar named Anti-Venom from the one possessing Groot to counteract the rest of its natural, extraterrestrial counterparts. After everyone on Earth is cured by Anti-Venom, Doctor Strange takes the Anti-Venomized Groot into space so that the Guardians of the Galaxy can be cured.

• Spider-Man / Peter Parker (voiced by Robbie Daymond)[7] - The show's teenage protagonist. After he was bitten by a genetically-modified spider during a field trip to Oscorp, Peter tries to balance his crime-fighting and civilian lives as a student in Horizon High and a freelance photographer at the Daily Bugle. Thus far, only Miles, Harry, Max, Octavius, Venom, Eddie, and Anna Maria know Spider-Man's secret identity.

• / Miles Morales / Ultimate Spider-Man (voiced by Nadji Jeter)[7] - One of Peter's classmates at Horizon High and primary partner-in-justice. After being bitten by the Electrolis Arachnatis spider, he is mentored by Spider-Man on how to use his abilities in "Ultimate Spider-Man". Miles is the first person to discover Spider-Man's secret identity, as shown in "Kraven's Amazing Hunt". In "Vengeance Of Venom" Pt. 2, Miles is among the heroes captured by the Klyntar during their invasion of Earth before being cured by Anti-Venom. Thus far, only Peter, Gwen, Anya and Octavius know Miles's secret identity.

• Ghost-Spider / Gwen Stacy / Spider-Gwen (voiced by Laura Bailey)[7] - One of Peter's classmates at Horizon High. Unique to this show, she is portrayed as Warren's niece. In Season 1, Gwen briefly takes on the Spider-Gwen alias when New York was exposed to the Jackal's chemicals during the "Spider-Island" arc. In Season 2, Gwen regains her abilities due to exposure to the Blood Gem relic and adopts the Ghost-Spider alias in "The Day Without Spider-Man". In "Vengeance Of Venom" Pt. 2, Ghost-Spider is among the heroes captured by the Klyntar during their invasion of Earth before being cured by Anti-Venom. Thus far, only Peter, Miles and Anya know Ghost-Spider's secret identity.

• Spider-Girl / Anya Corazon (voiced by Melanie Minichino)[7] - One of Peter's classmates at Horizon High and Gwen's best friend. In Season 1, Anya gains Spider abilities when the majority of New York was exposed to the Jackal's chemicals in the "Spider-Island" arc. In Season 2, Anya regains her Spider abilities due to the earlier experimental cure she took and adopts the Spider-Girl alias during the "Bring on the Bad Guys" arc. In "Vengeance Of Venom" Pt. 2, Spider-Girl is among the heroes captured by the Klyntar during their invasion of Earth before being cured by Anti-Venom. Thus far, only Gwen and Miles know Spider-Girl's secret identity.

• Hobgoblin / Harry Osborn (voiced by Max Mittelman)[7] - Peter's best friend and a student at Osborn Academy. Throughout Season 1, Harry was resentful of Spider-Man, blaming him for his expulsion from Horizon High, yet supportive towards Peter. Harry eventually discovers his best friend's secret identity during the "Spider Island" arc, putting his friendship with Peter at risk as he still blames Spider-Man for his misfortunes. In "The Hobgoblin" Pt. 1, Harry receives the Hobgoblin technology from Norman and reconciles with Peter after saving him from Octavius' mind control. In "The Hobgoblin" Pt. 2, Harry ultimately sides with Peter after seeing Norman's true colors. In Season 2, Harry spends a long vacation overseas running Oscorp as the company's acting CEO following Norman's presumed death. He eventually returns in the finale arc "Goblin War", where he assists the Spider Team in stopping the Goblin Nation. In "Vengeance Of Venom" Pt. 2, Hobglobin is among the heroes captured by the Klyntar during their invasion of Earth before being cured by Anti-Venom. Unique to this show, he is depicted with black hair compared to past series.

• Doctor Octopus / Otto Octavius / Living Brain / Superior Spider-Man (voiced by Scott Menville as Doctor Octopus, Robbie Daymond as Superior Spider-Man)[7] - A scientific 19-year-old prodigy who graduated from school early and became a teacher at Horizon High. Growing up, Octavius had a hard life between being picked on by a jock named Steve and having problems with his own father Torbert. In Season 1, an accident by Crimson Dynamo caused his harness with tentacle-like appendages to be fused to his body, turning him into a criminal, renaming himself "Doctor Octopus". He turns the Osborn Commandos into the Sinister Five yet his plans are foiled by Spider-Man and Hobgoblin. In Season 2, Octavius' body falls into a coma and his consciousness ends up in cyberspace to remain alive before relocating himself into Spider-Man's body, adopting the Superior Spider-Man alias. Despite his original agenda, Octavius restores himself and Peter to their former selves after seeing the errors of his ways. Octavius dies by fading away into light particles after stopping the Goblin King using the Neuro Cortex leaving his tentacles behind. He is honored as a hero by the rest of the Spider Team, who attend his funeral along with Anna Maria and Max.

• Max Modell (voiced by Fred Tatasciore)[7] - The headmaster of Horizon High who is a target of various antagonists' personal vendettas. Max eventually pieces together Peter's identity as Spider-Man in "Goblin War: Part 4", admitting this to Peter with the promise that he will keep it a secret in "Web of Venom" Pt. 1. As of "Web of Venom" Pt. 2, Max is placed under investigation by Dr. Connors for his "corrupt operations".

• Aunt May Parker (voiced by Nancy Linari)[7] - Peter's aunt and sole guardian following her husband Ben's death.

• Uncle Ben Parker (voiced by Patton Oswalt)[7] - Peter's late uncle and May's late husband.

• Flash Thompson (voiced by Benjamin Diskin) - A student jock at Midtown High and Osborn Academy. First appearing in "Osborn Academy", he initially picks on Peter and admires Spider-Man. Flash has traumatic encounters with the Venom symbiote in "Venom" and "Superior" but gets rescued by Spider-Man both times.

• Joe Q. (voiced by Joe Quesada) - The manager of a coffeehouse that is frequented by Peter and Harry.

• Randy Robertson (voiced by Zeno Robinson) - One of Peter's classmates at Midtown High. He first appears in the series premiere "Horizon High". In "How I Thwipped My Summer Vacation", Randy runs into Peter on the street and helps him get a job at the Daily Bugle by having his father hook him up with Jameson.

• Screwball / Liz Allan (voiced by Natalie Lander) - One of Peter's classmates at Midtown High who is ranked class president. She first appears in the series premiere "Horizon High". Liz also operates as the internet celebrity prankster Screwball but develops a change of heart after Spider-Man saved her and Randy from Absorbing Man and Hammerhead in "Screwball Live". Thus far, only Spider-Man and Randy know Screwball's secret identity.

• Mary Jane Watson (voiced by Felicia Day[8]) - One of Peter's classmates at Midtown High. After only making a brief cameo in "Venom", where she is dressed as the school's tiger mascot, she makes her full appearance in person in the Maximum Venom season.

• J. Jonah Jameson (voiced by Bob Joles)[9] - The Daily Bugle Communications' head, John's father, and the Spider Team's regular critic. Peter worked for Jameson as a freelance photographer, providing footage of Spider-Man that was in better quality compared to Eddie's, from "Take Two" until "Venom Returns", where he fires Peter for putting him, Gwen, Anya, May and Max in danger by aiding Spider-Man (actually Miles in disguise) in thwarting Venom and Eddie's hostage plot. He generally views Spider-Man as a menace but temporarily supported him when Octavius was Superior Spider-Man, being impressed with his more ruthless demeanor.

• Anna Maria Marconi (voiced by Katrina Kemp) - The dwarfish assistant chemistry teacher at Midtown High. In "Cloak and Dagger", she befriends Octavius (as Peter) and assists Superior Spider-Man in defeating Cloak and Dagger, as well as exposing Tiberius Stone's true colors. In "Superior", Anna Maria gets taken hostage by the Venom symbiote as a bargaining chip to undo Octavius' body-swap with Peter, leading her to discover Spider-Man's secret identity. After Peter defeats Venom, she learns the truth about Superior Spider-Man and declares Octavius a true hero. In "The Cellar", Anna Maria helps Spider-Man by freeing Dagger so that Cloak can rescue the Avengers. During Season 2's finale arc "Goblin War", Anna Maria assists Doctor Octopus in providing tactical support during the Spider Team's battles with the Goblin Nation.

• Grady Scraps (voiced by Scott Menville) - An intelligent student who formally attended Bilderberg Academy, which was used as a front by A.I.M. for their superhuman experiments in "School of Hard Knocks". After spending some time in home-school in light of Bilderberg Academy being shut down, Grady is enrolled at Horizon High in "Web of Venom" Pt. 1.

The Avengers are a world-renowned team of Earth's Mightiest Heroes, the Avengers occasionally lend the Spider Team a hand over the course of their adventures. They also have young heroes-in-training such as Ironheart and Totally Awesome Hulk working under their guidance and training to become veteran heroes.

• Iron Man / Tony Stark (voiced by Mick Wingert) - The Avengers' benefactor and CEO of Stark Industries who uses mechanical suits of armor of his own making. In "Stark Expo", Iron Man interacts with Spider-Man during the Stark Expo. In "School of Hard Knocks", Iron Man can be seen rounding up the captured A.I.M. operatives. In "A Troubled Mind", Iron Man is among the Avengers investigating Miles' suspicions of Superior Spider-Man. In "Brand New Day" and "The Cellar", Iron Man is among the Avengers imprisoned in another dimension by Regent and are rescued by Spider-Man, Octavius, Anna Maria, Cloak, and Dagger before helping the former defeat Regent. In "Vengeance of Venom", Iron Man is among the Avengers captured by the Klyntar while on a mission in space before being cured by Anti-Venom.

• Hulk / Bruce Banner (voiced by Fred Tatasciore as Hulk, Kevin Shinick as Bruce Banner) - A genius scientist who, because of exposure to gamma radiation, typically transforms into a monster when enraged or agitated. In "Halloween Moon", Hulk helps Spider-Man, Gwen, and Harry cure John Jameson of his Man-Wolf mutation. In "School of Hard Knocks," Hulk is among the Avengers taken prisoner by A.I.M. for experimentation and rescued by Spider-Man and Ms. Marvel. In "Brand New Day" and "The Cellar", Hulk is among the Avengers imprisoned in another dimension by Regent and are rescued by Spider-Man, Octavius, Anna Maria, Cloak, and Dagger before helping the former defeat Regent.

• Black Widow / Natasha Romanova (voiced by Laura Bailey) - A highly trained spy and agent of S.H.I.E.L.D.. Unique to this show, she is depicted to have a short mohawk hairstyle. In "Spider Island" Pt. 2, Black Widow teams up with Spider-Man and Spider-Gwen in stopping Hydra's operation run by Crossbones. In "School of Hard Knocks", Black Widow can be seen rounding up the captured A.I.M. operatives. In "A Troubled Mind", Black Widow is among the Avengers investigating Miles' suspicions of Superior Spider-Man. In "Brand New Day" and "The Cellar", Black Widow is among the Avengers imprisoned in another dimension by Regent and are rescued by Spider-Man, Octavius, Anna Maria, Cloak, and Dagger before helping the former defeat Regent.

• Ms. Marvel / Kamala Khan (voiced by Kathreen Khavari) - A teenage Muslim-American and an Inhuman with the ability to alter her size, shape, and form. In "School of Hard Knocks", Ms. Marvel teams up with Spider-Man to rescue Hulk, Captain America, and Captain Marvel from A.I.M. In "A Troubled Mind", Ms. Marvel is among the Avengers investigating Miles' suspicions of Superior Spider-Man. In "Brand New Day" and "The Cellar", Ms. Marvel is among the Avengers imprisoned in another dimension by Regent and are rescued by Spider-Man, Octavius, Anna Maria, Cloak, and Dagger before helping the former defeat Regent.

• Captain America / Steve Rogers (voiced by Roger Craig Smith) - A patriotic superhero from World War II who was enhanced to the peak of human physicality by an experimental serum and frozen in suspended animation before waking up decades later in the modern world. In "School of Hard Knocks", Captain America is among the Avengers taken prisoner by A.I.M. for experimentation and rescued by Spider-Man and Ms. Marvel. In "Brand New Day" and "The Cellar", Captain America is among the Avengers imprisoned in another dimension by Regent and are rescued by Spider-Man, Octavius, Anna Maria, Cloak, and Dagger before helping the former defeat Regent. In "Vengeance of Venom", Captain America is among the Avengers captured by the Klyntar while on a mission in space before being cured by Anti-Venom.

• Captain Marvel / Carol Danvers - A superheroine whose DNA was altered in a past incident, granting her energy-based abilities. In "School of Hard Knocks", Captain Marvel is among the Avengers taken prisoner by A.I.M. for experimentation and rescued by Spider-Man and Ms. Marvel. In "Vengeance of Venom", Captain Marvel is among the Avengers captured by the Klyntar while on a mission in space before being cured by Anti-Venom.

• Thor - The crown prince of Asgard, based on the Norse mythological deity of the same name. In "Vengeance of Venom", Thor is among the Avengers captured by the Klyntar while on a mission in space before being cured by Anti-Venom.

• Ironheart / Riri Williams (voiced by Sofia Wylie) - A teenage super genius and inventor who created her own suit of armor utilizing the same technology as Iron Man. In "Amazing Friends", Ironheart helps Spider-Man protect and rescue Groot from A.I.M. long enough to decode his message regarding the Klyntar's invasion of Earth. In "Vengeance of Venom" Pt. 2, Ironheart is among the heroes captured by the Klyntar during their invasion of Earth before being cured by Anti-Venom.
  • "Not Tony" (voiced by Mick Wingert) - A Tony Stark-inspired A.I. created by Ironheart.

• Totally Awesome Hulk / Amadeus Cho (voiced by Ki Hong Lee) - The 7th smartest person on Earth who can transform himself into a Hulk-like being using a device worn on his wrist. According to himself, his Hulk form is much different than Dr. Banner's. In "Amazing Friends" Pt. 2, Totally Awesome Hulk helps Spider-Man and Ironheart rescue Groot from A.I.M. and Baron Mordo. In "Vengeance of Venom", Totally Awesome Hulk is among the heroes possessed by the Klyntar during the early stages of their invasion of Earth before being cured by Anti-Venom.

The Guardians of the Galaxy are a group of intergalactic adventures and bounty hunters who Spider-Man previously met in the Guardians of the Galaxy: Mission Breakout! episodes "Back in the New York Groove" and "Drive My Carnage".

• Star-Lord / Peter Quill (voiced by Will Friedle)[10] - The half-human half-Spartoi leader of the Guardians of the Galaxy. In "Amazing Friends," Star-Lord sends Groot to Earth to warn Spider-Man of the Klyntar's invasion of Earth being captured by one of the symbiotes.

• Groot (voiced by Kevin Michael Richardson as an adult, Connor Andrade as a toddler) - A member of the Guardians of the Galaxy who is a tree-like humanoid; in this series, he is currently in toddler stage. Spider-Man previously met Groot when he was in his baby stage. In "Amazing Friends" Pt. 1, Groot comes to Earth to warn Spider-Man about the symbiote invasion orchestrated by Venom only to be captured by A.I.M. In "Amazing Friends" Pt. 2, Groot is rescued by Spider-Man, Spy-D, Ironheart, Doctor Strange, and Totally Awesome Hulk and is able to relay his message. In "Vengeance of Venom" Pt 1, Groot is among the heroes captured by the Klyntar during the early stages of their invasion of Earth. In "Vengeance of Venom" Pt. 2, Max is able to capture the Venomized Groot so he can create Anti-Venom. Once that was done, Doctor Strange takes the Anti-Venomized Groot so that he can cure the rest of the Guardians of the Galaxy.

• Nocturnal (voiced by Yuri Lowenthal) - A young owl-like transhuman who acquired his current appearance during a lab accident and became a recluse from fear of how the world would judge him. In "How I Thwipped My Summer Vacation", Nocturnal lived out deep in the forest, where he stole food from people to survive. After Nocturnal steals food from his friends while they were on a camping trip, Spider-Man befriends Nocturnal and learns of his tragic past yet is unable to convince him to meet his friends.
• Cloak / Tyrone Johnson (voiced by Aubrey Joseph) - A metahuman teenager capable of teleporting in a shroud of Darkforce energy. In "Cloak & Dagger", he and Dagger assault Midtown High to exact vengeance on Tiberius on account of being behind their powers with Alchemax's experiments (Cloak was identified as Subject 338) and ruining their lives. During their battle with Superior Spider-Man, Cloak is thrown into a wall by Tiberius' force-field and knocked out. In "Brand New Day", Cloak is incarcerated in The Cellar and is one of the villains suspected by Spider-Man to have been involved in the Avengers' capture, though it turns out Regent was replicating his powers. In "The Cellar", Cloak reforms by helping Spider-Man battle Regent upon escaping from his cell before rescuing the missing Avengers from Regent's pocket dimension. In "Vengeance Of Venom", Cloak is among heroes captured by the Klyntar during the early stages of their invasion of Earth before being cured by Anti-Venom.
• Dagger / Tandy Bowen (voiced by Olivia Holt) - A metahuman teenager who can generate dagger-like constructs out of Lightforce energy and Cloak's partner. In "Cloak and Dagger", she and Cloak assault Midtown High to exact vengeance on Tiberius on account of being behind their powers with Alchemax's experiments (Dagger was identified as Subject 339) and ruining their lives. During their battle with Superior Spider-Man, Dagger is struck by Anna Maria using a chair before being defeated by Superior Spider-Man. In "Brand New Day", Dagger is incarcerated in The Cellar. In "The Cellar", Dagger reforms by helping Cloak rescue the missing Avengers from Regent's pocket dimension after being freed by Octavius and Anna Maria, allowing them to free the Avengers. In "Vengeance of Venom" Pt. 2, Dagger is among the heroes captured by the Klyntar during their invasion of Earth before being cured by Anti-Venom.
• Doctor Strange / Stephen Strange (voiced by Liam O'Brien) - The Sorcerer Supreme and a former neurosurgeon who gained magical and dimensional powers while recovering from after a prior car crash. In "Amazing Friends" Pt. 1, Miles seeks out Doctor Strange, hoping that he can help translate Groot's message, only for the two of them to be send to the Unknown Realms by Baron Mordo. In "Amazing Friends" Pt. 2, Doctor Strange and Miles manage to escape the Unknown Realms and, after A.I.M. and Mordo, are defeated, decodes Groot's warning of the Klyntar invasion. In "Vengeance of Venom" Pt. 1, Doctor Strange is captured by the Klyntar while trying to transport the symbiotes to an uninhabited dimension. In "Vengeance of Venom" Pt. 2, the Venomized Doctor Strange (referring to himself as the "Symbiote Supreme") reassembles the seed from Venom's meteor. Once he is cured by Anti-Venom, Doctor Strange takes the Anti-Venomized Groot to cure the rest of the Guardians of the Galaxy.
• Moon Knight / Marc Spector (voiced by Peter Giles) - A cynical survivalist who has given up his old hero identity as Moon Knight due to a personal tragedy that involved his family. In "Vengeance of Venom" Pt. 2, Spector is drawn out of retirement by Spider-Man after being missing-in-action for eight months to help him battle the Klyntar and find Max. Spector eventually reprises his Moon Knight alias after receiving a pep talk from Aunt May.

• Norman Osborn (voiced by Josh Keaton)[11] - Harry's father and Oscorp's original CEO. During Season 1, Norman secretly orchestrated his son's expulsion from Horizon High in the series premiere "Horizon High" in an attempt to make Harry as New York's definite hero, with Spider-Man's very existence being an interference with that vision. Although he was initially presumed dead, he resurfaces as the Spider King of the Man-Spider army during the "Spider-Island" arc. In Season 1's finale "Hobgoblin", Norman utilizes replica Hobgoblin technology so he can end Spider-Man himself but is ultimately betrayed by Harry and is presumed dead following an explosion.

• Jackal / Raymond Warren (voiсed by John DiMaggio)[7] - An animalistic, green-furred criminal. One of Season 1's central antagonists, he is portrayed as Gwen's uncle, and the creator of the spiders behind Peter's and Miles's Spider abilities. In his earlier life, Raymond was a former staff member at Empire State University who was dismissed for his genetic experiments. In addition to his specialty in hybridization, Raymond is capable of generating clones of himself, one served as a decoy in "Party Animals" to fool Spider-Man and the authorities into believing he's caught the real Jackal. During the "Rise of Doc Ock" arc, it is revealed that he conspired with Doctor Octopus and fully intended to use the genetically modified spiders to create an entire army at his command. His master plan unintentionally happened during the "Spider-Island" arc with the Man-Spider army, but was ultimately thwarted by the Spider-Men, Anya and Harry.

• Spencer Smythe (voiced by Benjamin Diskin) - A teacher at Midtown High School and an inventor of the Spider-Slayers who has a personal vendetta against Max. He first appears in the "Origin" shorts. In "Ultimate Spider-Man", Spencer has a rivalry between him and Alistair over capturing Spider-Kid is thwarted by Spider-Man and taken into police custody. It is also revealed Spencer has been collaborating with Jackal.
  • Alistair Smythe (voiced by Jason Spisak) - An inventor of the Spider-Slayers and Spencer's son. In "Party Animals", he auditioned for a scholarship at Osborn Academy by testing his Spider-Slayer against Spider-Man. During "The "Rise of Doc Ock", Alistair joins the Osborn Commandos before being brainwashed by Doctor Octopus and later freed by Hobgoblin in Season 1's finale "Hobgoblin". In "Goblin War: Part 1", Alistair takes Peter's position at Horizon High and is suspected by Spider-Man as the Goblin Nation's leader but is merely the organization's latest member.
  • Spider-Slayers - Spider-like robots that have been used by Alistair and Spencer to fight Spider-Man.

• Klyntar - A race of parasitic alien symbiote who can possess human hosts and turn them into hostile and aggressive entities with powers identical to Spider-Man's.
  • Venom / V-252 (voiced by Benjamin Diskin in "Venom", Ben Pronsky from "Dead Man's Party" until "Web of Venom" Pt. 2) - A volatile Klyntar scout sent to Earth to prepare the planet for invasion. In Season 1, the V-252 was sent over to Horizon High from the crash site of a meteor at Duncan Valley for research only for it to get stolen by Black Cat. The V-252 briefly merges with Spider-Man and later Flash as its host before removed by Spider-Man and taken into captivity. In Season 2, the V-252 merges with Eddie (who renames the symbiote "Venom") and secretly exploits his host's jealousy towards Peter. During an attempt to publicly expose Spider-Man's identity, Venom is put in a coma by an experimental sonic device, eventually reawakening from stasis when he is mutated into a blob-like monster unable to merge with a host. Assaulting Midtown High in search of Spider-Man, Venom discovers Superior Spider-Man's true colors upon finding Otto's consciousness in Spider-Man's body. After Octavius restores Peter's former self, Venom is overloaded into submission using electricity. In Maximum Venom, Venom is accidentally freed by Dr. Connors and uses an energy seed contained in his old meteor to summon the other Klyntar to Earth from his home planet. However, he is ultimately killed by Spider-Man, which is later confirmed by the Venomized Doctor Strange.

• Vulture / Adrian Toomes / Goblin King (voiced by Alistair Duncan)[7] - A vulture-themed villain and scientist. A personal vendetta against Max due to being fired for stealing equipment, he claims that the stolen tech was originally his. In Season 1, his first fight with Spider-Man in the series premiere "Horizon High" ended in defeat, but was released in Norman's custody to work at Osborn Academy in "Symbiotic Relationship" where he developed Vulture technology to be used by Oscorp as the Vulture Force. During "The Rise of Doc Ock", Vulture joins the Osborn Commandos before being brainwashed by Doctor Octopus and later freed by Hobgoblin in Season 1's finale "Hobgoblin". In Season 2, Adrian works to reform himself in "Rise Above It All" when he's recruited by the Wake Riders as their way of asking him to lead. During Season 2's finale arc "Goblin War", Adrian is revealed to have established the Goblin Nation, becoming its semi-charismatic leader the Goblin King, and manipulated Harry into constructing the Goblin Mech. Adrian is ultimately defeated when Doctor Octopus sabotages the Goblin Mech using the Neuro Cortex and is webbed up by Spider-Man shortly after ejecting.

• Scorpion / Mac Gargan (voiced by Jason Spisak) - A scorpion-themed villain. In the series premiere "Horizon High", Spider-Man stops Scorpion from robbing the science museum. In "Kraven's Amazing Hunt", Scorpion is used as bait to hunt the Spider-Men in an episode of Kraven's show. In "The Day Without Spider-Man", Scorpion teams up with Tinkerer in a bid to enhanced himself using the Blood Gem and draw out Spider-Man, only to be defeated by Miles, Spider-Girl, and Ghost-Spider.

• Black Cat / Felicia Hardy (voiced by Grey DeLisle) - A cat burglar who can cause bad luck. In "A Day in the Life", Spider-Man's first run-in with Black Cat inevitably leads to meeting the V-252. In "How I Thwipped my Summer Vacation", Black Cat battles and loses to Spider-Man during a trip to the beach.

• Rhino / Aleksei Sytsevich (voiced by Matthew Mercer, briefly voiced by Ben Pronsky in "Dead Man's Party") - A student at Horizon High who is constantly turned into a humanoid rhinoceros by Jackal. During "The Rise of Doc Ock", Rhino joins the Osborn Commandos before being brainwashed by Doctor Octopus and later freed by Hobgoblin in Season 1's finale "Hobgoblin".

• Sandgirl / Keemia Alvarado (voiced by Sofia Carson) - A teenage criminal with sand-related powers. She gained her abilities when Hammerhead dumped a mixture sand and toxic chemicals dumped on her. Blaming her father Flint Marko for what happened to her, Keemia partnered with Hammerhead (who became her mentor and father figure) and adopted the Sandgirl alias. In "Sandman", she fought and defeated Spider-Man and her father due to her great control over her abilities yet was beaten when Spider-Man merged with the V-252 and escaped. After a long absence, Sandgirl resurfaces in "Critical Update" where she begins forming her own criminal organization from the henchmen of Hammerhead and Silvermane following their bosses' defeats. However, she is challenged by Superior Spider-Man and Miles and gets electrocuted to the point of turning into glass. Prior to her capture, Miles prevents Superior Spider-Man from permanently shattering her. In "Brand New Day", Sandgirl (not longer frozen as glass) is incarcerated in The Cellar and is one of the villains suspected by Spider-Man to have been involved in the Avengers' capture, though it turns out Regent was replicating her powers.

• Hammerhead (voiced by Jim Cummings) - A hard-headed crime lord. In "Sandman", he is shown responsible for turning Flint Marko and Keemia into Sandman and Sandgirl and is taken into custody after being defeated by Spider-Man when bonded to the V-252 symbiote. In "Spider-Man on Ice", Hammerhead gets betrayed by his henchman Randy Macklin wanting to start his own crime spree. In "Screwball Live", Hammerhead and Absorbing Man went after Screwball and Randy in an attempt to draw out Spider-Man so they could reclaim a drive containing incriminating evidence on Hammerhead only for the two villains to be defeated. In "How I Thwipped my Summer Vacation", Hammerhead battles and loses to Spider-Man at a Ross Caliban concert. In "My Own Worst Enemy", Hammerhead is the first of several crime bosses defeated by Superior Spider-Man.

• Kraven the Hunter (voiced by Troy Baker) - A hunter with long hair and a cybernetic right arm with interests in hunting exotic animals on his TV show Kraven's Amazing Hunt. In "Kraven's Amazing Hunt", Kraven is hired by Norman to capture the Spider-Men for experimentation following the Spider-Slayers' repeated failures, only to betray Osborn and Vulture and be defeated by the two Spider-Men. In "Spider-Island" Pt. 3, Kraven allies with Peter, Anya and Harry in combating the first wave of Man-Spiders. When he unsuccessfully betrays the group by hunting the first two Man-Spiders (Gwen and Norman), Kraven is defeated once more.

• A.I.M. - An evil scientific organization. In "Symbiotic Relationship", Spider-Man thwarts a robbery with A.I.M. only to discover the V-252's true colors. In "School of Hard Knocks", A.I.M. used Bilderberg Academy as a front for a superhuman project overseen by Rappaccini in which they create superpowered children using Captain America, Hulk, and Captain Marvel for the experiments. In "My Own Worst Enemy", Silver Sable attempted to set off harmfully explosive A.I.M. caches in Central Park only to be stopped by Superior Spider-Man. In "A Troubled Mind", A.I.M. creates MODOK as their new leader only to be defeated by Superior Spider-Man, Iron Man, Black Widow, and Ms. Marvel. In "Amazing Friends", A.I.M. collaborated with Baron Mordo to capture Groot and use him as a template for mystical wood golems only to be stopped by Spider-Man, Miles, Ironheart, Doctor Strange, and Totally Awesome Hulk.
  • Monica Rappaccini (voiced by Grey DeLisle) - A.I.M.'s Scientist Supreme. In Season 2, Monica posed as the headmistress of an elite boarding school called Bilderberg Academy in "School of Hard Knocks". In "Amazing Friends," Monica collaborated with Baron Mordo to create mystical Wood Golems using Groot as a template.
  • MODOK (voiced by Charlie Adler) - A cybernetically-enhanced lifeform whose name is short for Mental Organism Designed Only for Kaos. He is A.I.M.'s current leader and was created during "A Troubled Mind".
  • A.I.M. Agents (Various Voices) - A.I.M.'s foot soldiers.

• Lizard / Dr. Curt Connors (voiced by Yuri Lowenthal) - A scientist at Oscorp who is subjected to the lizard formula. Norman Osborn turns Connors into a humanoid lizard until Spider-Man managed to cure him in "The Rise of Doc Ock" Pt. 2. In Maximum Venom, Dr. Connors is hired as Horizon High's bio-mechanical teacher and takes control of Max's lab after Max is put under investigation while working with unknown allies who have heard of Max's synthetic Venom symbiote project.

• Crossbones / Brock Rumlow (voiced by Fred Tatasciore) - A mercenary that works for Hydra. In "Spider-Island: Part 2", Crossbones led Hydra's operation involving Vibranium only to be driven back by Spider-Man, Spider-Gwen and Black Widow. In "Goblin War: Part 3", Crossbones is seen with the Goblin Nation as the War Goblins' leader. However, he gets into a scuffle with the Electro Goblins and weakens Electro with a device before being attacked by Ghost-Spider and defeated by Miles.

• Tinkerer (voiced by Aaron Abrams) - A criminal inventor and technician. In "How I Thwipped My Summer Vacation", Tinkerer battles and loses to Spider-Man at a baseball game. In "Brain Drain", Tinkerer attempted to claim the bounty placed on Spider-Man only to be defeated once more. In "The Day Without Spider-Man", Tinkerer teams up with Scorpion and created a weapon powered by the Blood Gem to enhance Scorpion. However, the weapon unknowingly reawakens Gwen's spider powers and their plot is thwarted by Miles, Spider-Girl and Ghost-Spider.

• Eddie Brock (voiced by Ben Pronsky) - A former employee at the Daily Bugle and a temporary host of the Venom symbiote. Growing jealous of Peter's progress, Eddie ends up bonding with Venom in "Dead Man's Party", discovering Spider-Man's secret identity. In "Venom Returns", Eddie is an accomplice in Venom's hostage plot and attempt to publicly expose Spider-Man's identity, though he gets himself fired by Jameson in the process. During Venom's battle against Spider-Man and Miles, Eddie is affected by the same experimental sonic device that puts Venom into a coma. In "Superior", Eddie is still comatose when Venom is mutated and escaped; it is also revealed by Venom that he exploited Eddie's jealously of Peter to fulfill his own plans for revenge.

• Silver Sable (voiced by April Stewart) - The leader of the Wild Pack mercenary group. In "Take Two", Sable and her team attempted to steal the Neuro Cortex before being defeated by Spider-Man and Doctor Octopus. It is later revealed in "Between an Ock and a Hard Place" that the Wild Pack was hired by Doctor Octopus. In "My Own Worst Enemy", Silver Sable attempts to set off explosive A.I.M. caches in Central Park only to be stopped by Superior Spider-Man.

• Electro (voiced by Daisy Lightfoot) - A female African-American teenage supervillain with electrical abilities. In "Bring on the Bad Guys" Pt. 4, Electro attempted to claim the bounty on Spider-Man but was defeated, with the destruction caused by her powers resulting in the Horizon High Open House being cancelled. In "The Living Brain", Electro battles Spider-Man and Chief Watanabe while imprisoned at the Supervillain Holding Facility, only to be defeated and returned to her cell. In "Brand New Day", Electro is incarcerated in The Cellar and is one of the villains suspected by Spider-Man to have been involved in the Avengers' capture, though it turns out Regent was replicating her powers. In "Goblin War: Part 3", Electro is seen with the Goblin Nation as the Electro Goblins' leader. During a scuffle with the War Goblins, she is weakened by a device used by Crossbones.

• Silvermane (voiced by Nolan North) - A crime lord named for his silver-colored hair. In "Bring on the Bad Guys" Pt. 3, Silvermane sent Prowler to capture Spider-Man so that he could obtain the bounty in exchange for releasing Abraham Brown after botching an assignment. However, Spider-Man and Prowler worked together to save Abraham from Silvermane. In "Critical Update", Silvermane is one of the crime bosses defeated by Superior Spider-Man. In "Goblin War: Part 3", Silvermane is seen with the Goblin Nation as the Cyber Goblins' leader and is eventually defeated once more by Spider-Man and Hobgoblin.

• Beetle / Abner Jenkins (voiced by Fred Tatasciore) - A beetle-themed criminal. In "Dead Man's Party", Beetle targets an armored truck using Oscorp's stolen technology only to be defeated by Spider-Man. In "The Living Brain", Beetle battles Spider-Man and Chief Watanabe while imprisoned at the Supervillain Holding Facility, only to be defeated and returned to his cell.

• Tiberius Stone (voiced by Jonathan Brooks) - Alchemax's CEO and a former benefactor of Midtown High. In "Cloak and Dagger", it is revealed that Tiberius was behind Cloak's and Dagger's powers with his experiments. After a battle with Superior Spider-Man, he is taken into custody and Anna Maria withdraws Midtown High's sponsorship proposal to Alchemax.

• Molten Man (voiced by Imari Williams) - A supervillain with fire abilities. In "Brand New Day", he is incapacitated by Mister Negative using a special sword. Recovering in time to crash Mister Negative's battle with Spider-Man in retaliation for the Inner Demons being on "his turf", he is defeated by Spider-Man and incarcerated at the Cellar. Molten Man is later one of the villains suspected by Spider-Man to have been involved in the Avengers' capture, though it turns out Regent was replicating his powers.

• Mister Negative / Martin Li (voiced by Eric Bauza) - A supervillain with Darkforce powers and the leader of the Inner Demons criminal gang. In "Brand New Day", he incapacitates Molten Man using a special sword before being defeated by Spider-Man and incarcerated at the Cellar. Mister Negative is later one of the villains suspected by Spider-Man to have been involved in the Avengers' capture, though it turns out Regent was replicating his powers.

• Regent / Augustus Roman (voiced by Imari Williams) - The Cellar prison's warden and Watanabe's mentor. As Regent, he wears a special suit capable of tapping into the powers of the Cellar's inmates, namely Cloak, Dagger, Electro, Mister Negative, Molten Man, Sandgirl, Spot, Frostbite, Whirlwind and Paradox. Roman held a vendetta because the Avengers arrested his father for what he saw as only a few felonies where he gained a life sentence. In "Brand New Day", he banishes Iron Man, Hulk, Captain America, Ms. Marvel, Black Widow, and Spider-Man into another dimension. In "The Cellar", Regent's plan to keep the Avengers imprisoned in his pocket dimension is undone by Cloak and Dagger, who had been liberated by Spider-Man, Doctor Octopus and Anna Maria. Regent is ultimately defeated by Spider-Man and the Avengers before being relieved of his duty as warden and remanded to the Cellar, which Jameson reports on.

• Goblin Nation - A cult-like organization led by the Goblin King. They appear throughout Season 2's finale arc "Goblin War", using colored variants of the Goblin technology. In addition to Toomes, Barkley, the Wake Riders, Electro, Crossbones, Silvermane, Dr. Rockwell, and Alistair, the organization also includes:
  • Goblin Thugs (Various Voices) - The Goblin Nation's common foot soldiers. As different variations of Goblin Thugs exist, they wear recolored Goblin armors that depend on the type of weapons they utilize.
    • Electro Goblins - A variation of Goblin Thugs equipped with electricity-emitting gauntlets that are under Electro's command. They first appear in "Goblin War: Part 2."
    • Cyber Goblins - A cybernetically-enhanced variation of Goblin Thugs that are under Silvermane's command. They first appear in "Goblin War: Part 3."
    • War Goblins - A variation of Goblin Thugs equipped with missile launchers that are under Crossbones's command. They first appear in "Goblin War: Part 3."

• Technovore - A technology-eating monstrosity that grows upon how much technology it eats. In the Maximum Venom premiere "Web of Venom", the Technovore was released from Horizon High's project graveyard by Dr. Connors, who made it look like it was an accident caused by Grady Scraps, before being stopped by Spider-Man using the synthetic Venom copy.

• Baron Mordo (voiced by Leonard Roberts) - A rouge Master of the Mystic Arts and an enemy of Doctor Strange. In "Amazing Friends" Pt. 1, Mordo banishes Doctor Strange and Miles into the Unknown Realms. In "Amazing Friends", Pt. 2, it is revealed that Mordo was collaborating with A.I.M. to create a legion of mystical wood golems based off Groot. He is defeated by Spider-Man, Miles, Ironheart, Doctor Strange, and Totally Awesome Hulk.

• In Guardians of the Galaxy: Mission Breakout, Spider-Man makes guest appearances in the episodes "Back in the New York Groove" and "Drive My Carnage", with Max Modell and Venom also appearing in the latter episode.
• In Avengers: Black Panther's Quest, Kraven the Hunter makes a guest appearance in the episode "T'Challa Royale", and Spider-Man and Vulture later make guest appearances in the episode "The Vibranium Curtain, Part 2".

In October 2016, the series was announced by Cort Lane, senior vice president of Marvel Animation, as a replacement for the series' predecessor, Ultimate Spider-Man, which ended its run in early January 2017. It premiered on August 19, 2017 on Disney XD.[5][12]

The "Maximum Venom" season debuted on April 19, 2020.[8]

• Amanda Goodbread – Casting Director and Recording Manager
• Philip Pignotti – Supervising Director[12]
• Kevin Shinick – Story Editor[12]
• Collette Sunderman – ADR Voice Director (Season One), Voice Director (Season Two)
• Kris Zimmerman-Salter – Voice Director (Season One)