TRNA (guanine26-N2)-dimethyltransferase

TRNA (guanine26-N2)-dimethyltransferase
Identifiers
EC number 2.1.1.216
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

TRNA (guanine26-N2)-dimethyltransferase (EC 2.1.1.216, Trm1p, TRM1, tRNA (m22G26)dimethyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (guanine26-N2)-dimethyltransferase.[1][2][3][4] This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + guanine26 in tRNA 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA

The enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions.

References

  1. ↑ Constantinesco, F.; Motorin, Y.; Grosjean, H. (1999). "Characterisation and enzymatic properties of tRNA(guanine26, N2,N2-dimethyltransferase (Trm1p) from Pyrococcus furiosus". J. Mol. Biol. 291: 375–392. doi:10.1006/jmbi.1999.2976. PMID 10438627.
  2. ↑ Constantinesco, F.; Benachenhou, N.; Motorin, Y.; Grosjean, H. (1998). "The tRNA(guanine-26,N2-N2) methyltransferase (Trm1) from the hyperthermophilic archaeon Pyrococcus furiosus: cloning, sequencing of the gene and its expression in Escherichia coli". Nucleic Acids Res. 26: 3753–3761. doi:10.1093/nar/26.16.3753. PMC 147764. PMID 9685492.
  3. ↑ Liu, J.; Liu, J.; Straby, K.B. (1998). "Point and deletion mutations eliminate one or both methyl group transfers catalysed by the yeast TRM1 encoded tRNA (m22G26)dimethyltransferase". Nucleic Acids Res. 26: 5102–5108. doi:10.1093/nar/26.22.5102. PMC 147968. PMID 9801306.
  4. ↑ Liu, J.; Zhou, G.Q.; Straby, K.B. (1999). "Caenorhabditis elegans ZC376.5 encodes a tRNA (m22G26)dimethyltransferance in which 246arginine is important for the enzyme activity". Gene. 226: 73–81. doi:10.1016/s0378-1119(98)00550-2. PMID 10048958.
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