RRNA small subunit pseudouridine methyltransferase Nep1

RRNA small subunit pseudouridine methyltransferase Nep1
Identifiers
EC number 2.1.1.260
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

RRNA small subunit pseudouridine methyltransferase Nep1 (EC 2.1.1.260, Nep1, nucleolar essential protein 1) is an enzyme with systematic name S-adenosyl-L-methionine:18S rRNA (pseudouridine1191-N1)-methyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA

This enzyme recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal RNA based on the local RNA structure.

References

  1. ↑ Taylor, A.B.; Meyer, B.; Leal, B.Z.; Kötter, P.; Schirf, V.; Demeler, B.; Hart, P.J.; Entian, K.D.; Wöhnert, J. (2008). "The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site". Nucleic Acids Res. 36: 1542–1554. doi:10.1093/nar/gkm1172. PMC 2275143. PMID 18208838.
  2. ↑ Wurm, J.P.; Meyer, B.; Bahr, U.; Held, M.; Frolow, O.; Kötter, P.; Engels, J.W.; Heckel, A.; Karas, M.; Entian, K.D.; Wöhnert, J. (2010). "The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase". Nucleic Acids Res. 38: 2387–2398. doi:10.1093/nar/gkp1189. PMC 2853112. PMID 20047967.
  3. ↑ Meyer, B.; Wurm, J.P.; Kötter, P.; Leisegang, M.S.; Schilling, V.; Buchhaupt, M.; Held, M.; Bahr, U.; Karas, M.; Heckel, A.; Bohnsack, M.T.; Wöhnert, J.; Entian, K.D. (2011). "The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA". Nucleic Acids Res. 39: 1526–1537. doi:10.1093/nar/gkq931. PMC 3045603. PMID 20972225.
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.