Crossover junction endodeoxyribonuclease

Crossover junction endodeoxyribonuclease
Identifiers
EC number 3.1.22.4
CAS number 99676-43-4
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Crossover junction endodeoxyribonuclease (EC 3.1.22.4, Hje endonuclease, Holliday junction endonuclease CCE1, Holliday junction resolvase, Holliday junction-cleaving endonuclease, Holliday junction-resolving endoribonuclease, RusA Holliday junction resolvase, RusA endonuclease, RuvC endonuclease, SpCCe1 Holliday junction resolvase, crossover junction endoribonuclease, cruciform-cutting endonuclease, endo X3, endonuclease RuvC, endonuclease VII, endonuclease X3, resolving enzyme CCE1) is an enzyme.[1][2][3][4][5] This enzyme catalyses the following chemical reaction

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)

The enzyme from Saccharomyces cerevisiae is not active on DNA molecules that do not contain Holliday junctions.

References

  1. ↑ Symington LS, Kolodner R (November 1985). "Partial purification of an enzyme from Saccharomyces cerevisiae that cleaves Holliday junctions". Proceedings of the National Academy of Sciences of the United States of America. 82 (21): 7247–51. doi:10.1073/pnas.82.21.7247. PMC 390826. PMID 3903750.
  2. ↑ Shida T, Iwasaki H, Saito A, Kyogoku Y, Shinagawa H (October 1996). "Analysis of substrate specificity of the RuvC holliday junction resolvase with synthetic Holliday junctions". The Journal of Biological Chemistry. 271 (42): 26105–9. doi:10.1074/jbc.271.42.26105. PMID 8824253.
  3. ↑ Shah R, Cosstick R, West SC (March 1997). "The RuvC protein dimer resolves Holliday junctions by a dual incision mechanism that involves base-specific contacts". The EMBO Journal. 16 (6): 1464–72. doi:10.1093/emboj/16.6.1464. PMC 1169743. PMID 9135161.
  4. ↑ Fogg JM, Schofield MJ, White MF, Lilley DM (August 1999). "Sequence and functional-group specificity for cleavage of DNA junctions by RuvC of Escherichia coli". Biochemistry. 38 (35): 11349–58. doi:10.1021/bi990926n. PMID 10471285.
  5. ↑ Middleton CL, Parker JL, Richard DJ, White MF, Bond CS (January 2003). "Crystallization and preliminary X-ray diffraction studies of Hje, a HolliDay junction resolving enzyme from Sulfolobus solfataricus". Acta Crystallographica D. 59 (Pt 1): 171–3. doi:10.1107/s0907444902019546. PMID 12499561.
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