23S rRNA (adenine2503-C8)-methyltransferase

23S rRNA (adenine2503-C8)-methyltransferase
Identifiers
EC number 2.1.1.224
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

23S rRNA (adenine2503-C8)-methyltransferase (EC 2.1.1.224, Cfr (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenine2503-C8)-methyltransferase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA

This enzyme is a member of the 'AdoMet radical' (radical SAM) family.

References

  1. ↑ Giessing, A.M.; Jensen, S.S.; Rasmussen, A.; Hansen, L.H.; Gondela, A.; Long, K.; Vester, B.; Kirpekar, F. (2009). "Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria". RNA. 15: 327–336. doi:10.1261/rna.1371409. PMC 2648713. PMID 19144912.
  2. ↑ Kaminska, K.H.; Purta, E.; Hansen, L.H.; Bujnicki, J.M.; Vester, B.; Long, K.S. (2010). "Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria". Nucleic Acids Res. 38: 1652–1663. doi:10.1093/nar/gkp1142. PMC 2836569. PMID 20007606.
  3. ↑ Yan, F.; LaMarre, J.M.; Röhrich, R.; Wiesner, J.; Jomaa, H.; Mankin, A.S.; Fujimori, D.G. (2010). "RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA". J. Am. Chem. Soc. 132: 3953–3964. doi:10.1021/ja910850y. PMC 2859901. PMID 20184321.
  4. ↑ Yan, F.; Fujimori, D.G. (2011). "RNA methylation by Radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift". Proc. Natl. Acad. Sci. USA. 108 (10): 3930–3934. doi:10.1073/pnas.1017781108. PMC 3054002. PMID 21368151.
  5. ↑ Grove, T.L.; Benner, J.S.; Radle, M.I.; Ahlum, J.H.; Landgraf, B.J.; Krebs, C.; Booker, S.J. (2011). "A radically different mechanism for S-adenosylmethionine-dependent methyltransferases". Science. 332 (6029): 604–607. doi:10.1126/science.1200877. PMID 21415317.
  6. ↑ Boal, A.K.; Grove, T.L.; McLaughlin, M.I.; Yennawar, N.H.; Booker, S.J.; Rosenzweig, A.C. (2011). "Structural basis for methyl transfer by a radical SAM enzyme". Science. 332 (6033): 544–545. doi:10.1126/science.1205358. PMC 3506250. PMID 21527678.
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