D-Man-alpha-(1-3)-D-Glc-beta-(1-4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase

D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase
Identifiers
EC number 2.4.1.264
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase (EC 2.4.1.264, GumK) is an enzyme with systematic name UDP-glucuronate:D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol beta-1,2-glucuronyltransferase.[1][2][3][4][5][6][7] This enzyme catalyses the following chemical reaction

UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol UDP + D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol

The enzyme is involved in the biosynthesis of the exopolysaccharides xanthan (in the bacterium Xanthomonas campestris) and acetan (in the bacterium Gluconacetobacter xylinus).

References

  1. ↑ Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Puhler, A.; Ielpi, L. (1998). "Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence". J. Bacteriol. 180 (7): 1607–1617. PMC 107069. PMID 9537354.
  2. ↑ Ielpi, L.; Couso, R.O.; Dankert, M.A. (1993). "Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris". J. Bacteriol. 175 (9): 2490–2500. PMC 204549. PMID 7683019.
  3. ↑ Kim, S.Y.; Kim, J.G.; Lee, B.M.; Cho, J.Y. (2009). "Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae pv oryzae". Biotechnol. Lett. 31 (2): 265–270. doi:10.1007/s10529-008-9858-3. PMID 18854951.
  4. ↑ Barreras, M.; Bianchet, M.A.; Ielpi, L. (2006). "Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis". Acta Crystallographica Section F. 62 (Pt 9): 880–883. doi:10.1107/S1744309106028764. PMC 2242869. PMID 16946469.
  5. ↑ Barreras, M.; Salinas, S.R.; Abdian, P.L.; Kampel, M.A.; Ielpi, L. (2008). "Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase". J. Biol. Chem. 283 (36): 25027–25035. doi:10.1074/jbc.M801227200. PMC 3259810. PMID 18596046.
  6. ↑ Vojnov, A.A.; Bassi, D.E.; Daniels, M.J.; Dankert, M.A. (2002). "Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris". Carbohydr. Res. 337 (4): 315–326. doi:10.1016/S0008-6215(01)00322-6. PMID 11841812.
  7. ↑ Barreras, M.; Abdian, P.L.; Ielpi, L. (2004). "Functional characterization of GumK, a membrane-associated β-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis". Glycobiology. 14 (3): 233–241. doi:10.1093/glycob/cwh056. PMID 14736729.
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