Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)
| bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.6.1.17 | ||||||||
| CAS number | 37289-29-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) (EC 3.6.1.17) is an enzyme that catalyzes the chemical reaction
- P1,P4-bis(5'-guanosyl) tetraphosphate + H2O GTP + GMP
Thus, the two substrates of this enzyme are P1,P4-bis(5'-guanosyl) tetraphosphate and H2O, whereas its two products are GTP and GMP.
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is P1,P4-bis(5'-nucleosyl)-tetraphosphate nucleotidohydrolase. Other names in common use include bis(5'-guanosyl)-tetraphosphatase, bis(5'-adenosyl)-tetraphosphatase, diguanosinetetraphosphatase (asymmetrical), dinucleosidetetraphosphatase (asymmetrical), diadenosine P1,P4-tetraphosphatase, dinucleoside tetraphosphatase, and 1-P,4-P-bis(5'-nucleosyl)-tetraphosphate nucleotidohydrolase. This enzyme participates in purine metabolism and pyrimidine metabolism.
Structural studies
As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1F3Y, 1JKN, 1KT9, 1KTG, 1XSA, 1XSB, and 1XSC.
References
- Jakubowski H, Guranowski A (1983). "Enzymes hydrolyzing ApppA and/or AppppA in higher plants Purification and some properties of diadenosine triphosphatase, diadenosine tetraphosphatase, and phosphodiesterase from yellow lupin (Lupinus luteus) seeds". J. Biol. Chem. 258 (16): 9982&ndash, 9. PMID 6309793.
- Vallejo CG, Lobaton CD, Quintanilla M, Sillero A, Sillero MA (1976). "Dinucleosidasetetraphosphatase in rat liver and Artemia salina". Biochim. Biophys. Acta. 438 (1): 304&ndash, 9. doi:10.1016/0005-2744(76)90246-1. PMID 181087.
- Warner AH, Finamore FJ (1965). "Isolation, purification, and characterization of P1,P4-diguanosine 5'-tetraphosphate asymmetrical-pyrophosphohydrolase from brine shrimp eggs". Biochemistry. 4 (8): 1568&ndash, 75. doi:10.1021/bi00884a016. PMID 4955726.