List of Animaniacs characters

• Yakko (voiced by Rob Paulsen) Yakko is a loquacious, wise-cracking smart-aleck, who usually acts as the leader of the trio. He is also obsessed with girls, and is known for saying "Goodnight, everybody!" after a joke that only adult viewers will understand. Yakko wears tan pants with a black belt.
• Wakko (voiced by Jess Harnell) – Wakko has a Liverpudlian accent, a huge appetite and a "gag bag" full of tricks. He also appears to be the least intelligent, or most insane, although Yakko has claimed it to be "middle kid syndrome" in the episode "Survey Ladies". Wakko wears a red backwards baseball cap and a light blue turtleneck sweater. He is also very talented at burping out songs during "The Great Wakkorotti" segments (the burps were provided by Maurice LaMarche)
• Dot (voiced by Tress MacNeille) – Dot is cute, sassy and more easily relaxed than her brothers, but proves on numerous occasions that she can be just as zany. Her full name is "Princess Angelina Contessa Louisa Francesca Banana Fanna Bo Besca III". She despises being referred to as "Dottie", threatening "Call me Dottie and you die." She wears a pink skirt with a flower in her ears. While no writer or creator has confirmed the fact, it has been suggested that Dot's name comes from the period, or 'dot', found at the end of 'Warner Bros.'.

Pinky and the Brain are two white mice kept in a cage at ACME Labs, voiced by Rob Paulsen and Maurice LaMarche, respectively. The Brain is serious and clearly the leader, and constantly devises plans to conquer the world; Pinky is eccentric and unintelligent, but loyal to the Brain. In 1995, they were spun off into a cartoon series of their own.

• Slappy Squirrel – Grumpy cartoon veteran, voiced by series writer Sherri Stoner impersonating Penny Marshall, who lives in a tree with nephew Skippy Squirrel.[4] The music played during her segments is an excerpt from Antonín Dvořák's "Humoresque No. 7".
• Skippy Squirrel – Voiced by Nathan Ruegger, Skippy's chipper personality is the opposite of his aunt's. His character varies, from slightly naive ("Slappy Goes Walnuts") to innocent ("Bumbie's Mom") to complicit partner of Slappy ("Critical Condition").

The Goodfeathers are an Italian American trio of pigeons: Squit (gray), Bobby (turquoise), and Pesto (lavender), who were voiced by Maurice LaMarche, John Mariano and Chick Vennera, influenced by Ray Liotta, Robert De Niro, and Joe Pesci's roles in Goodfellas.

These segments, along with "Minerva Mink", were discontinued at the end of Season 1 (in part, because of Bernadette Peters' salary). Welker remained a series regular, voicing other characters. Rita and Runt returned as minor characters toward the series' end.

Rita and Runt are a vagabond stray duo that get into so many scraps and adventures. They are often searching for a home, but are back as strays by the end of the episode.

• Rita (voiced by Bernadette Peters, with vocal effects by Frank Welker) - Rita is a funny, aloof and intelligent cat that sings.
• Runt (voiced by Welker) - Runt is a dim-witted dog who thinks that Rita is also a dog like himself and who constantly uses the word, "definitely" a lot when speaking (for example, "Rita, you're a good dog. Definitely a good dog!"). This verbal tic, as well as Runt's speaking style, is a reference to Dustin Hoffman's character in Rain Man.

Buttons is a German Shepard who watches Mindy when her parents are away and is voiced by Frank Welker. Mindy, voiced by Nancy Cartwright, is a young girl who constantly wanders into trouble without being aware of it. Buttons haplessly struggles with various dangers and narrowly rescues Mindy, only to be blamed and punished for her misbehavior. There is no consistency or continuity in the storytelling; most episodes each feature Mindy's family living in a different setting and portraying them as different sorts of people (I.E. costumed superheroes, mer-people, cave people, etc.).

Minerva Mink is an attractive young mink, voiced by Julie Brown, who was called Marilyn Mink in pre-production.

• The Baby Bluebird ("Birdie") – Voiced by Cody Ruegger.
• Chicken Boo – Six-foot-tall chicken, voiced by Frank Welker. He wishes to live as a human, so he wears flimsy disguises, usually just a hat or a coat, which somehow always fool everyone (except for one person who no one believes). Unlike other animal characters, Boo cannot talk and acts almost exactly like a real chicken, making his disguises all the more absurd. At the end of each episode, his disguise falls apart, and he is exposed as a giant chicken. This causes all of his previous supporters to turn against him by saying "I told you that guy was a chicken!" and usually running him out of town.
• Steven Spielberg (voiced by Frank Welker) – The show's executive producer, often mentioned by the cast.
• Colin (the Randy Beaman Kid) – Wide-eyed boy who tells improbable stories which allegedly happened to his (never-seen) friend, Randy Beaman. He comes out of his home with an object in hand that he fidgets with while he tells the story (the object is usually not related to the story) and starts off with "One time...okay, see, one time..." and then tells his story while playing with whatever he has. When he is finished, something will usually happen with the object that he is playing with (like an ice cream cone melting or a baseball going through someone's window), and the kid will finish with "'kay, bye." and walk back into his house.
• The Flame – Childlike candle flame, voiced by Luke Ruegger, who is present at important historical events and teaches fire safety.
• Flavio and Marita – Also known as the Hip Hippos, a wealthy, Spanish hippo couple voiced by Frank Welker and Tress MacNeille. (Singing voices done by Wendy Knudsen for Marita, and Ray McLeod for Flavio.)
• Dr. Gina Embryo – Zoologist who studies the Hip Hippos and tries in vain to protect them, a parody of Joan Embery and Jane Goodall voiced by Tress MacNeille.
• Mr. Cory Skullhead – Mute skeleton seen in the "Good Idea-Bad Idea" segment narrated by Tom Bodett and a parody of Edward Scissorhands.
• The Mime – Nameless mime who appears in "Mime Time", also narrated by Bodett.
• Katie Ka-Boom – A teenage girl voiced by Laura Mooney,[5] who morphs into various violent, destructive monsters when things do not go her way. She lives with her parents and her little brother named Tinker. Katie is the only member of her family who has blonde hair while the rest of her family are brunettes. The premiere Katie Ka-boom segment was also a Chicken Boo crossover, in which Katie morphs into a green Incredible Hulk-like monster when initially told her new boyfriend (who is also the student council president, class valedictorian and the school's star wrestler) is a giant chicken, and then morphs into a monster made of fire when she herself realizes that he is a giant chicken and ends their relationship.
• Mary Hartless – Parody of Mary Hart, voiced by Valri Bromfield and Tress MacNeille, who appears as a newsreader with a variety of hairstyles in "Hurray for Slappy", "Chairman of the Bored", "Bubba Bo Bob Brain", and "Critical Condition".
• Death – Archetypical Grim Reaper with black robe, skeletal appearance, scythe and speaks in a Swedish accent. In "Meatballs or Consequences" the Warner siblings challenge him to a game of checkers, reminiscent of the chess game with Death in Ingmar Bergman's 1956 The Seventh Seal.[6][7]
• The Narrator – Offscreen narrator (voiced by Jim Cummings) whose voice resembles Cummings' voice for Winnie the Pooh, who appears in "Nighty-Night Toon", "Gift of Gold", and "Warners and the Beanstalk".
• Francis "Pip" Pumphandle (voiced by Ben Stein) a man who tells long and boring stories, and bores the Warners in "Chairman of the Bored". He also appears in the Pinky and the Brain episode "Star Warners", and returns in Wakko's Wish as a Desire Fulfillment Facilitator.
• Bugs Bunny - a bunny who appears in Looney Tunes.
• Daffy Duck - a duck who appears in Looney Tunes.