tRNA (guanine-N1-)-methyltransferase
| tRNA (guanine-N1-)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.1.1.31 | ||||||||
| CAS number | 37256-99-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a tRNA (guanine-N1-)-methyltransferase (EC 2.1.1.31) is an enzyme that catalyzes the chemical reaction
- S-adenosyl-L-methionine + tRNA S-adenosyl-L-homocysteine + tRNA containing N1-methylguanine
Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N1-methylguanine.
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N1-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 1-methyltransferase, tRNA guanine 1-methyltransferase, and S-adenosyl-L-methionine:tRNA (guanine-1-N-)-methyltransferase.
Structural studies
As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1OY5, 1P9P, 1UAJ, 1UAK, 1UAL, and 1UAM.
References
- Hurwitz J, Gold M, Anders M (1964). "The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. 3. Purification of Soluble Ribonucleic Acid-Methylating Enzymes". J. Biol. Chem. 239: 3462&ndash, 3473. PMID 14245404.
- Bjork GR, Svensson I (1969). "Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae". Eur. J. Biochem. 9 (2): 207&ndash, 215. doi:10.1111/j.1432-1033.1969.tb00596.x. PMID 4896260.
- Smolar N, Hellman U, Svensson I (1975). "Two transfer RNA (1-methylguanine) methylases from yeast". Nucleic Acids Res. 2 (6): 993&ndash, 1004. doi:10.1093/nar/2.6.993. PMC 343485. PMID 1096087.
- Glick JM, Averyhart VM, Leboy PS (1978). "Purification and characterization of two tRNA-(guanine)-methyltransferases from rat liver". Biochim. Biophys. Acta. 518 (1): 158&ndash, 171. doi:10.1016/0005-2787(78)90125-9. PMID 629973.