Rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,3/1,4-galactofuranosyltransferase

Rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,3/1,4-galactofuranosyltransferase
Identifiers
EC number 2.4.1.287
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,3/1,4-galactofuranosyltransferase (EC 2.4.1.287, arabinogalactan galactofuranosyl transferase 1, GlfT1) is an enzyme with systematic name UDP-alpha-D-galactofuranose:alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 3-beta/4-beta-galactofuranosyltransferase.[1][2] This enzyme catalyses the following chemical reaction

2 UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 2 UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol (overall reaction)
(1a) UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol UDP + beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
(1b) UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol

This enzyme is isolated from Mycobacterium tuberculosis and M. smegmatis.

References

  1. ↑ Mikusová, K.; Belánová, M.; Korduláková, J.; Honda, K.; McNeil, M.R.; Mahapatra, S.; Crick, D.C.; Brennan, P.J. (2006). "Identification of a novel galactosyl transferase involved in biosynthesis of the mycobacterial cell wall". J. Bacteriol. 188 (18): 6592–6598. doi:10.1128/JB.00489-06. PMC 1595490. PMID 16952951.
  2. ↑ Belánová, M.; Dianisková, P.; Brennan, P.J.; Completo, G.C.; Rose, N.L.; Lowary, T.L.; Mikusová, K. (2008). "Galactosyl transferases in mycobacterial cell wall synthesis". J. Bacteriol. 190 (3): 1141–1145. doi:10.1128/JB.01326-07. PMC 2223555. PMID 18055597.
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