Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase

Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase
Identifiers
EC number 2.4.2.43
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase (EC 2.4.2.43, undecaprenyl phosphate-alpha-L-Ara4N transferase, 4-amino-4-deoxy-L-arabinose lipid A transferase, polymyxin resistance protein PmrK, arnT (gene)) is an enzyme with systematic name 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate:lipid IVA 4-amino-4-deoxy-L-arabinopyranosyltransferase.[1][2][3][4][5] This enzyme catalyses the following chemical reaction

(1) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans,octacis-undecaprenyl phosphate
(2) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + lipid IVA lipid IIA + ditrans,octacis-undecaprenyl phosphate
(3) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA 4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + ditrans,octacis-undecaprenyl phosphate

This integral membrane protein is present in the inner membrane of certain Gram negative endobacteria.

References

  1. ↑ Trent, M.S.; Ribeiro, A.A.; Lin, S.; Cotter, R.J.; Raetz, C.R. (2001). "An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor". J. Biol. Chem. 276: 43122–43131. doi:10.1074/jbc.m106961200. PMID 11535604.
  2. ↑ Trent, M.S.; Ribeiro, A.A.; Doerrler, W.T.; Lin, S.; Cotter, R.J.; Raetz, C.R. (2001). "Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm". J. Biol. Chem. 276: 43132–43144. doi:10.1074/jbc.m106962200. PMID 11535605.
  3. ↑ Zhou, Z.; Ribeiro, A.A.; Lin, S.; Cotter, R.J.; Miller, S.I.; Raetz, C.R. (2001). "Lipid A modifications in polymyxin-resistant Salmonella typhimurium: PMRA-dependent 4-amino-4-deoxy-L-arabinose, and phosphoethanolamine incorporation". J. Biol. Chem. 276: 43111–43121. doi:10.1074/jbc.m106960200. PMID 11535603.
  4. ↑ Bretscher, L.E.; Morrell, M.T.; Funk, A.L.; Klug, C.S. (2006). "Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium". Protein Expr. Purif. 46: 33–39. doi:10.1016/j.pep.2005.08.028. PMID 16226890.
  5. ↑ Impellitteri, N.A.; Merten, J.A.; Bretscher, L.E.; Klug, C.S. (2010). "Identification of a functionally important loop in Salmonella typhimurium ArnT". Biochemistry. 49: 29–35. doi:10.1021/bi901572f. PMC 2805049. PMID 19947657.
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