Glycoside hydrolase family 45

Glycosyl hydrolase family 45
endoglucanase from humicola insolens at 1.7a resolution
Identifiers
Symbol Glyco_hydro_45
Pfam PF02015
Pfam clan CL0199
InterPro IPR000334
PROSITE PDOC00877
SCOP 2eng
SUPERFAMILY 2eng
CAZy GH45

In molecular biology, glycoside hydrolase family 45 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]

Glycoside hydrolase family 45 CAZY GH_45 comprises enzymes with only one known activity; endoglucanase (EC 3.2.1.4). This family is also known as cellulase family K.[8] The best conserved region in these enzymes is located in the N-terminal section. It contains an aspartic acid residue which has been shown[9] to act as a nucleophile in the catalytic mechanism. This also has several cysteines that are involved in forming disulphide bridges.

References

  1. ↑ Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America. 92 (15): 7090–4. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
  2. ↑ Davies G, Henrissat B (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–9. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
  3. ↑ Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal. 316 ( Pt 2) (Pt 2): 695–6. PMC 1217404. PMID 8687420.
  4. ↑ "Home". CAZy.org. Retrieved 2018-03-06.
  5. ↑ Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research. 42 (Database issue): D490–5. doi:10.1093/nar/gkt1178. PMID 24270786.
  6. ↑ "Glycoside Hydrolase Family 45". CAZypedia.org. Retrieved 2018-03-06.
  7. ↑ "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes". Glycobiology. 28 (1): 3–8. December 2018. doi:10.1093/glycob/cwx089. PMID 29040563.
  8. ↑ Henrissat B, Bairoch A (August 1993). "New families in the classification of glycosyl hydrolases based on amino acid sequence similarities". The Biochemical Journal. 293 ( Pt 3): 781–8. doi:10.1042/bj2930781. PMC 1134435. PMID 8352747.
  9. ↑ Davies GJ, Dodson GG, Hubbard RE, Tolley SP, Dauter Z, Wilson KS, Hjort C, Mikkelsen JM, Rasmussen G, Schülein M (September 1993). "Structure and function of endoglucanase V". Nature. 365 (6444): 362–4. doi:10.1038/365362a0. PMID 8377830.
This article incorporates text from the public domain Pfam and InterPro: IPR000334
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