Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase

Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase
Identifiers
EC number 2.4.1.288
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase (EC 2.4.1.288, GlfT2) is an enzyme with systematic name UDP-alpha-D-galactofuranose:beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/5-beta-D-galactofuranosyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction

28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 28 UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol

This enzyme is solated from Mycobacterium tuberculosis.

References

  1. ↑ Rose, N.L.; Zheng, R.B.; Pearcey, J.; Zhou, R.; Completo, G.C.; Lowary, T.L. (2008). "Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase". Carbohydr. Res. 343 (12): 2130–2139. doi:10.1016/j.carres.2008.03.023. PMID 18423586.
  2. ↑ May, J.F.; Splain, R.A.; Brotschi, C.; Kiessling, L.L. (2009). "A tethering mechanism for length control in a processive carbohydrate polymerization". Proc. Natl. Acad. Sci. USA. 106 (29): 11851–11856. Bibcode:2009PNAS..10611851M. doi:10.1073/pnas.0901407106. PMC 2715488. PMID 19571009.
  3. ↑ Wheatley, R.W.; Zheng, R.B.; Richards, M.R.; Lowary, T.L.; Ng, K.K. (2012). "Tetrameric structure of GlfT2 reveals a scaffold for the assembly of mycobacterial arabinogalactan". J. Biol. Chem. 287 (33): 28132–43. doi:10.1074/jbc.M112.347484. PMC 3431634. PMID 22707726.
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