Exo-1,4-beta-D-glucosaminidase

Exo-1,4-beta-D-glucosaminidase
Identifiers
EC number 3.2.1.165
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Exo-1,4-beta-D-glucosaminidase (EC 3.2.1.165, CsxA, GlcNase, exochitosanase, GlmA, exo-beta-D-glucosaminidase, chitosan exo-1,4-beta-D-glucosaminidase) is an enzyme with systematic name chitosan exo-(1->4)-beta-D-glucosaminidase.[1][2][3]![4][5] This enzyme catalyses the following chemical reaction

Hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini

Chitosan is a partially or totally N-deacetylated chitin derivative that is found in the cell walls of some phytopathogenic fungi .

References

  1. ↑ Nanjo F, Katsumi R, Sakai K (June 1990). "Purification and characterization of an exo-beta-D-glucosaminidase, a novel type of enzyme, from Nocardia orientalis". The Journal of Biological Chemistry. 265 (17): 10088–94. PMID 2351651.
  2. ↑ Nogawa M, Takahashi H, Kashiwagi A, Ohshima K, Okada H, Morikawa Y (March 1998). "Purification and Characterization of Exo-beta-d-Glucosaminidase from a Cellulolytic Fungus, Trichoderma reesei PC-3-7". Applied and Environmental Microbiology. 64 (3): 890–5. PMC 106342. PMID 16349528.
  3. ↑ Fukamizo T, Fleury A, Côté N, Mitsutomi M, Brzezinski R (November 2006). "Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism". Glycobiology. 16 (11): 1064–72. doi:10.1093/glycob/cwl026. PMID 16877749.
  4. ↑ Côté N, Fleury A, Dumont-Blanchette E, Fukamizo T, Mitsutomi M, Brzezinski R (March 2006). "Two exo-beta-D-glucosaminidases/exochitosanases from actinomycetes define a new subfamily within family 2 of glycoside hydrolases". The Biochemical Journal. 394 (Pt 3): 675–86. doi:10.1042/bj20051436. PMC 1383717. PMID 16316314.
  5. ↑ Ike M, Isami K, Tanabe Y, Nogawa M, Ogasawara W, Okada H, Morikawa Y (October 2006). "Cloning and heterologous expression of the exo-beta-D-glucosaminidase-encoding gene (gls93) from a filamentous fungus, Trichoderma reesei PC-3-7". Applied Microbiology and Biotechnology. 72 (4): 687–95. doi:10.1007/s00253-006-0320-y. PMID 16636831.
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