Eagle's minimal essential medium

Eagle's minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture.

It contains:

A variation of this medium, called Dulbecco's modified Eagle's medium (DMEM), (Dulbecco/Vogt modified Eagle's minimal essential medium), contains approximately four times as much of the vitamins and amino acids present in the original formula and two to four times as much glucose. Additionally, it contains iron and phenol red. DMEM is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish[1] cells.

α-MEM (Minimum Essential Medium Eagle - alpha modification) is a medium based on EMEM. It contains more non-essential amino acids, sodium pyruvate, and vitamins compared with EMEM.[2][3]

See also

  • RPMI (Roswell Park Memorial Institute medium), for lymph cells
  • GMEM (Glasgow's Minimal Essential Medium)

References

  1. ↑ Pombinho AR, Laizé V, Molha DM, Marques SMP, Cancela ML (March 2004). "Development of two bone-derived cell lines from the marine teleost Sparus aurata; evidence for extracellular matrix mineralization and cell-type-specific expression of matrix Gla protein and osteocalcin". Cell and Tissue Research. 315 (3): 393–406. doi:10.1007/s00441-003-0830-1. PMID 14762709.
  2. ↑ "α-MEM" (PDF).
  3. ↑ "Alpha MEM with Nucleosides". Stem Cell Tech.


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