25-hydroxycholesterol 7α-hydroxylase

25-hydroxycholesterol 7alpha-hydroxylase
Identifiers
EC number 1.14.13.100
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

25-hydroxycholesterol 7alpha-hydroxylase (EC 1.14.13.100, 25-hydroxycholesterol 7alpha-monooxygenase, CYP7B1, CYP7B1 oxysterol 7alpha-hydroxylase) is an enzyme with systematic name cholest-5-ene-3beta,25-diol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating).[1][2][3][4] This enzyme catalyses the following chemical reaction

(1) cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 cholest-5-ene-3beta,7alpha,25-triol + NADP+ + H2O
(2) cholest-5-ene-3beta,27-diol + NADPH + H+ + O2 cholest-5-ene-3beta,7alpha,27-triol + NADP+ + H2O

25-hydroxycholesterol 7α-hydroxylase is a heme-thiolate protein (P-450).

References

  1. ↑ Toll, A.; Wikvall, K.; Sudjana-Sugiaman, E.; Kondo, K.H.; Björkhem, I. (1994). "7α hydroxylation of 25-hydroxycholesterol in liver microsomes. Evidence that the enzyme involved is different from cholesterol 7α-hydroxylase". Eur. J. Biochem. 224: 309–316. doi:10.1111/j.1432-1033.1994.00309.x. PMID 7925343.
  2. ↑ Li-Hawkins, J.; Lund, E.G.; Bronson, A.D.; Russell, D.W. (2000). "Expression cloning of an oxysterol 7α-hydroxylase selective for 24-hydroxycholesterol". J. Biol. Chem. 275: 16543–16549. doi:10.1074/jbc.M001810200. PMID 10748047.
  3. ↑ Ren, S.; Marques, D.; Redford, K.; Hylemon, P.B.; Gil, G.; Vlahcevic, Z.R.; Pandak, W.M. (2003). "Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the rat". Metabolism. 52: 636–642. doi:10.1053/meta.2003.50106. PMID 12759897.
  4. ↑ Russell, D.W. (2003). "The enzymes, regulation, and genetics of bile acid synthesis". Annu. Rev. Biochem. 72: 137–174. doi:10.1146/annurev.biochem.72.121801.161712. PMID 12543708.
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