Laminar flow cabinet

A laminar flow cabinet or tissue culture hood is a carefully enclosed bench designed to prevent contamination of semiconductor wafers, biological samples, or any particle sensitive materials. Air is drawn through a HEPA filter and blown in a very smooth, laminar flow towards the user. Due to the direction of air flow, the sample is protected from the user but the user is not protected from the sample. The cabinet is usually made of stainless steel with no gaps or joints where spores might collect.[1]

Laminar flow cabinet
Laminar flow hood
UsesParticulate removal
Related itemsBiosafety cabinet
Fume hood
Preparation of microbiological samples in a laminar chamber.

Such hoods exist in both horizontal and vertical configurations, and there are many different types of cabinets with a variety of airflow patterns and acceptable uses.

Laminar flow cabinets may have a UV-C germicidal lamp to sterilize the interior and contents before usage to prevent contamination of the experiment. Germicidal lamps are usually kept on for fifteen minutes to sterilize the interior before the cabinet is used. The light must be switched off when the cabinet is being used, to limit exposure to skin and eyes as stray ultraviolet light emissions can cause cancer and cataracts.[2]

See also

  1. Primary Containment for Biohazards: Selection, Installation and Use of Biological Safety Cabinets 3rd Edition Sept. 2007 USA CDC
  2. NSF/ANSI Standard 49

References

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