Xiaoliang Sunney Xie

Xiaoliang Sunney Xie
Born (1962-06-24) June 24, 1962
Beijing, China
Education BSc in Chemistry from Peking University
PhD in Physical Chemistry from UC San Diego
Known for Single Molecule Enzymology,
Coherent Raman Imaging,
Single Cell Genomics
Awards Albany Prize in Medicine and Biomedical Research 2015,
Peter Debye Award in Physical Chemistry 2015,
Ellis R. Lippincott Award 2013,
Biophysical Society Founders Award 2012
Scientific career
Fields Biophysical Chemistry,
Optical Imaging,
Genomics
Institutions Harvard University
Peking University
Doctoral advisor John Douglas Simon[1]

Professor Xiaoliang Sunney Xie (Chinese: 谢晓亮; born 1962 in Beijing, China) is considered a founding father of single-molecule biophysical chemistry and single-molecule enzymology.[2] He received his B.Sc. in Chemistry from Peking University in 1984, and his Ph.D. in Physical Chemistry in 1990 from University of California at San Diego. After a brief postdoctoral appointment at University of Chicago, he joined Pacific Northwest National Laboratory, where he rose from Senior Research Scientist to Chief Scientist. In 1998, he became the first tenured professor at Harvard University among Chinese Scholars who came to the United States since the Reform in China.

As a pioneer of single-molecule biophysical chemistry, coherent Raman scattering microscopy, and single-cell genomics, he made major contributions to the emergence of these fields. Furthermore, he has made significant advances on medical applications of label-free optical imaging and single-cell genomics. In particular, his inventions in single-cell genomics have been used in in vitro fertilization to benefit hundreds of couples in China by avoiding the transmission of monogenic diseases to their newborns.

Currently, Xie is the Mallinckrodt Professor of Chemistry and Chemical Biology at Harvard University. He is also the Director of Biodynamics Optical Imaging Center (BIOPIC) since 2010, and the Director of Beijing Advanced Innovation Center for Genomics (ICG) since 2016, both at Peking University.

As of April 2017, Xie has published over 210 papers. More than forty of his students and post-doctorates have become professors at major universities around the world, and two are co-founders of start-up companies.

Professor Xie’s current research interests include the following scientific, technological, and medical areas:

Honors and awards

Selected Literature

Single-Cell Genomics

  • Chen, Chongyi; Xing, Dong; Tan, Longzhi; Li, Heng; Zhou, Guangyu; Huang, Lei; Xie, X. Sunney (2017). "Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI)". Science. 356: 189–194. Bibcode:2017Sci...356..189C. doi:10.1126/science.aak9787.
  • Yan, Liying; Huang, Lei; Xu, Liya; Huang, Jin; Ma, Fei; Zhu, Xiaohui; Tang, Yaqiong; Liu, Mingshan; Lian, Ying; Liu, Ping; Li, Rong; Lu, Sijia; Tang, Fuchou; Qiao, Jie; Xie, X Sunney (2015). "Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses". Proc Natl Acad Sci USA. 112 (52): 15964–15969. Bibcode:2015PNAS..11215964Y. doi:10.1073/pnas.1523297113.
  • Hou, Yu; Fan, Wei; Yan, Liying; Li, Rong; Lian, Ying; Huang, Jin; Li, Jinsen; Xu, Liya; Tang, Fuchou; Xie, X. Sunney; Qiao, Jie (2013). "Genome Analyses of Single Human Oocytes". Cell. 155: 1492–1506. doi:10.1016/j.cell.2013.11.040.
  • Zong, Chenghang; Lu, Sijia; Chapman, Alec R.; Xie, X. Sunney (2012). "Genome-Wide Detection of Single-Nucleotide and Copy-Number Variations of a Single Human Cell". Science. 338: 1622–1626. Bibcode:2012Sci...338.1622Z. doi:10.1126/science.1229164.

Gene Expression and Regulation

  • Chong, Shasha; Chen, Chongyi; Ge, Hao; Sunney, Xie X. (2014). "Mechanism of Transcriptional Bursting in Bacteria". Cell. 158: 314–326. doi:10.1016/j.cell.2014.05.038.
  • Taniguchi, Yuichi; Choi, Paul J.; Li, Gene-Wei; Chen, Huiyi; Babu, Mohan; Hearn, Jeremy; Emili, Andrew; Xie, X. Sunney (2010). "Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells". Science. 329: 533–538. Bibcode:2010Sci...329..533T. doi:10.1126/science.1188308. PMC 2922915.
  • Choi, Paul J.; Cai, Long; Frieda, Kirsten; Xie, X. Sunney (2008). "A Stochastic Single-Molecule Event Triggers Phenotype Switching of a Bacterial Cell". Science. 322: 442–446. Bibcode:2008Sci...322..442C. doi:10.1126/science.1161427.
  • Elf, Johan; Li, Gene-Wei; Xie, X. Sunney (2007). "Probing Transcription Factor Dynamics at the Single-Molecule Level in a Living Cell". Science. 316: 1191–1194. Bibcode:2007Sci...316.1191E. doi:10.1126/science.1141967. PMC 2853898.
  • Yu, Ji; Xiao, Jie; Ren, Xiaojia; Lao, Kaiqin; Xie, X. Sunney (2006). "Probing Gene Expression in Live Cells, One Protein Molecule at a Time". Science. 311: 1600–1603. Bibcode:2006Sci...311.1600Y. doi:10.1126/science.1119623.
  • Cai, Long; Friedman, Nir; Xie, X. Sunney (2006). "Stochastic Protein Expression in Individual Cells at the Single Molecule Level". Nature. 440: 358–362. Bibcode:2006Natur.440..358C. doi:10.1038/nature04599.

Single Molecule Enzymology

  • Chong, Shasha; Chen, Chongyi; Ge, Hao; Sunney, Xie X. (2014). "Mechanism of Transcriptional Bursting in Bacteria". Cell. 158: 314–326. doi:10.1016/j.cell.2014.05.038.
  • Kim, Sangjin; Broströmer, Erik; Xing, Dong; Jin, Jianshi; Chong, Shasha; Ge, Hao; Wang, Siyuan; Gu, Chan; Yang, Lijiang; Gao, Yiqin; Su, Xiao-dong; Sun, Yujie; Xie, X. Sunney (2013). "Probing Allostery Through DNA". Science. 339: 816–819. Bibcode:2013Sci...339..816K. doi:10.1126/science.1229223.
  • English, Brian P.; Min, Wei; van Oijen, Antoine M.; Lee, Kang Taek; Luo, Guobin; Sun, Hongye; Cherayil, Binny J.; Kou, S.C.; Xie, X. Sunney (2006). "Ever-Fluctuating Single Enzyme Molecules: Michaelis-Menten Equation Revisited". Nat Chem Biol. 2: 87–94.
  • Yang, Haw; Luo, Guobin; Karnchanaphanurach, Pallop; Louie, Tai-Man; Rech, Ivan; Cova, Sergio; Xun, Luying; Xie, X. Sunney (2003). "Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer". Science. 302: 262–266. Bibcode:2003Sci...302..262Y. doi:10.1126/science.1086911.
  • Lu, H. Peter; Xun, Luying; Xie, X. Sunney (1998). "Single-Molecule Enzymatic Dynamics". Science. 282: 1877–1882. doi:10.1126/science.282.5395.1877.

Coherent Raman Imaging

  • Ji, Minbiao; Orringer, Daniel A.; Freudiger, Christian W.; Ramkissoon, Shakti; Liu, Xiaohui; Lau, Darryl; Golby, Alexandra J.; Norton, Isaiah; Hayashi, Marika; Agar, Nathalie Y. R.; Young, Geoffrey S.; Spino, Cathie; Santagata, Sandro; Camelo-Piragua, Sandra; Ligon, Keith L.; Sagher, Oren; Xie, X. Sunney (2013). "Rapid, Label-Free Detection of Brain Tumors with Stimulated Raman Scattering Microscopy". Science Translational Medicine. 5: 201. doi:10.1126/scitranslmed.3005954.
  • Wang, Meng C.; Min, Wei; Freudiger, Christian W.; Ruvkun, Gary; Xie, X. Sunney (2011). "RNAi Screening for Fat Regulatory Genes with SRS Microscopy". Nat Methods. 8: 135–138. doi:10.1038/nmeth.1556. PMC 3061290.
  • Saar, Brian G.; Freudiger, Christian W.; Reichman, Jay; Stanley, C. Michael; Holtom, Gary R.; Xie, X. Sunney (2010). "Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering". Science. 330: 1368–1370. Bibcode:2010Sci...330.1368S. doi:10.1126/science.1197236.
  • Freudiger, Christian W.; Min, Wei; Saar, Brian G.; Lu, Sijia; Holtom, Gary R.; He, Chengwei; Tsai, Jason C.; Kang, Jing X.; Xie, X. Sunney (2008). "Label-Free Biomedical Imaging with High sensitivity by Stimulated Raman Scattering Microscopy". Science. 322: 1857–1860. Bibcode:2008Sci...322.1857F. doi:10.1126/science.1165758.
  • Evans, Conor L.; Potma, Eric O.; Puoris'Haag, Mehron; Côté, Daniel; Lin, Charles P.; Xie, X. Sunney (2005). "Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy". Proc. Natl. Acad. Sci. 102: 16807–16812. Bibcode:2005PNAS..10216807E. doi:10.1073/pnas.0508282102. PMC 1283840.
  • Zumbusch, Andreas; Holtom, Gary R.; Xie, X. Sunney (1999). "Vibrational Microscopy Using Coherent Anti-Stokes Raman Scattering". Phys Rev Lett. 82: 4142–4145. Bibcode:1999PhRvL..82.4142Z. doi:10.1103/physrevlett.82.4142.

Single Molecule Imaging

  • Zhou, Yuexin; Wang, Ping; Tian, Feng; Gao, Ge; Huang, Lei; Wei, Wensheng; Xie, X. Sunney (2017). "Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging". Cell Res. 27: 298–301. doi:10.1038/cr.2017.9.
  • Chong, Shasha; Min, Wei; Xie, X. Sunney (2010). "Ground-State Depletion Microscopy:Detection Sensitivity of Single-Molecule Optical Absorption at Room Temperature". J Phys Chem Lett. 1: 3316–3322. doi:10.1021/jz1014289.
  • Min, Wei; Lu, Sijia; Chong, Shasha; Roy, Rahul; Holtom, Gary R.; Xie, X. Sunney (2009). "Imaging Chromophores with Undetectable Fluorescence by Stimulated Emission Microscopy". Nature. 461: 1105–1109. Bibcode:2009Natur.461.1105M. doi:10.1038/nature08438.
  • Sánchez, Erik J.; Novotny, Lukas; Xie, X. Sunney (1999). "Near-field Fluorescence Microscopy Based on Two-photon Excitation with Metal Tips". Phys Rev Lett. 82: 4014–4017. Bibcode:1999PhRvL..82.4014S. doi:10.1103/physrevlett.82.4014.
  • Sánchez, Erik J.; Novotny, Lukas; Holtom, G.R.; Xie, X. Sunney (1997). "Room-Temperature Fluorescence Imaging and Spectroscopy of Single Molecules by Two-Photon Excitation". J Phys Chem A. 101: 7019–7023. Bibcode:1997JPCA..101.7019S. doi:10.1021/jp971915b.
  • Xie, X. Sunney; Dunn, Robert C. (1994). "Probing Single Molecule Dynamics". Science. 265: 361–364. Bibcode:1994Sci...265..361X. doi:10.1126/science.265.5170.361.

References

  1. ↑ https://bernstein.harvard.edu/pages/AboutProfXie.html
  2. ↑ "Sunney Xie to receive Leibinger Research Prize for laser technology". Physics Today. 2013. doi:10.1063/pt.4.1557.
  3. ↑ http://newsen.pku.edu.cn/news_events/news/focus/6288.htm
  4. ↑ "关于公布2017年中国科学院院士增选当选院士名单的公告" (in Chinese). Chinese Academy of Sciences. 2017-11-28. Retrieved 2018-06-17.
  5. ↑ National Academy of Medicine Elects 79 New Members
  6. ↑ Albany Medical Center Prize
  7. ↑ Peter Debye Award
  8. ↑ NIH Director's Pioneer Award 2013
  9. ↑ Ellis R. Lippincott Award
  10. ↑ Berthold Leibinger Zukunftspreis
  11. ↑ Biography of Xie from the Website of the Willis E. Lamb Award (displayed June 4, 2008)
  12. ↑ NIH Director's Pioneer Award 2004
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