Small Cajal body specific RNA 17
| small Cajal body-specific RNA 17 | |
|---|---|
 Predicted secondary structure and sequence conservation of SCARNA17 | |
| Identifiers | |
| Symbol | SCARNA17 |
| Alt. Symbols | snoU12-22 |
| Rfam | RF00492 |
| Other data | |
| RNA type | Gene; snRNA; snoRNA; scaRNA |
| Domain(s) | Eukaryota |
| GO | 0006396 0015030 0005730 |
| SO | 0000275 |
| PDB structures | PDBe |
Small Cajal body-specific RNA 17 (also known as U12-22 scaRNA) is a type of small nuclear RNA which localises to the cajal bodies and proposed to guide the modification of RNA polymerase II transcribed spliceosomal RNAs U1, U2, U4, U5 and U12.[1][2]
The complete human U12-22/U4-8 scaRNA is composed of two tandem C/D box domains (termed U12-22 and U4-8). The 5' and 3' C/D domains are predicted to guide the 2'O-ribose methylation of residue U22 in U12 and residue C8 in U4 snRNAs respectively.[1] This family includes only the 5' C/D box domain (U12-22) as the 3' C/D box is represented by Small Cajal body specific RNA 18. The 3' C/D domain (U4-8) was also cloned previously by Darzacq and called U91.[3] Both the doublet (U12-22/U4-8) and singlet (U4-8) forms of this snRNA have been purified from HeLa cells. [1] The doublet form U12-22/U4-8 has been shown to localise to the nucleoplasm and is proposed to reside in the Cajal bodies whereas the U4-8 single domain appears to accumulate in the nucleolus. [1] In humans the genomic location of U12-88/U4-8 is intergenic and the purified transcript has been shown to possess a methylated guanosine cap suggesting it is independently transcribed by RNA polymerase II.[1]
References
- 1 2 3 4 5 Tycowski, Kazimierz T.; Aab, Alar; Steitz, Joan A. (23 November 2004). "Guide RNAs with 5′ Caps and Novel Box C/D snoRNA-like Domains for Modification of snRNAs in Metazoa". Current Biology. 14 (22): 1985–1995. doi:10.1016/j.cub.2004.11.003. PMID 15556860.
- ↑ Lestrade, Laurent; Weber, Michel J. (2006). "snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs". Nucleic Acids Research. 34 (Supplement 1: Database Issue): D158–D162. CiteSeerX 10.1.1.105.7552. doi:10.1093/nar/gkj002. PMC 1347365. PMID 16381836.
- ↑ Darzacq, Xavier; Jády, Beáta E.; Verheggen, Céline; Kiss, Arnold M.; Bertrand, Edouard; Kiss, Tamás (2002). "Cajal body-specific small nuclear RNAs: A novel class of 2'-O-methylation and pseudouridylation guide RNAs". The EMBO Journal. 21 (11): 2746–2756. doi:10.1093/emboj/21.11.2746. PMC 126017. PMID 12032087.
External links