Putidaredoxin—NAD+ reductase

Putidaredoxin—NAD+ reductase
Identifiers
EC number 1.18.1.5
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Putidaredoxin—NAD+ reductase (EC 1.18.1.5, putidaredoxin reductase, camA (gene)) is an enzyme with systematic name putidaredoxin:NAD+ oxidoreductase.[1][2][3][4][5][6][7] This enzyme catalyses the following chemical reaction

reduced putidaredoxin + NAD+ oxidized putidaredoxin + NADH + H+

Putidaredoxin—NAD+ reductase requires FAD.

References

  1. Roome, P.W.; Jr.; Philley, J.C.; Peterson, J.A. (1983). "Purification and properties of putidaredoxin reductase". J. Biol. Chem. 258: 2593–2598. PMID 6401738.
  2. Koga, H.; Yamaguchi, E.; Matsunaga, K.; Aramaki, H.; Horiuchi, T. (1989). "Cloning and nucleotide sequences of NADH-putidaredoxin reductase gene (camA) and putidaredoxin gene (camB) involved in cytochrome P-450cam hydroxylase of Pseudomonas putida". J. Biochem. 106: 831–836. PMID 2613690.
  3. Peterson, J.A.; Lorence, M.C.; Amarneh, B. (1990). "Putidaredoxin reductase and putidaredoxin. Cloning, sequence determination, and heterologous expression of the proteins". J. Biol. Chem. 265: 6066–6073. PMID 2180940.
  4. Sevrioukova, I.F.; Poulos, T.L. (2002). "Putidaredoxin reductase, a new function for an old protein". J. Biol. Chem. 277: 25831–25839. doi:10.1074/jbc.m201110200. PMID 12011076.
  5. Sevrioukova, I.F.; Garcia, C.; Li, H.; Bhaskar, B.; Poulos, T.L. (2003). "Crystal structure of putidaredoxin, the [2Fe-2S] component of the P450cam monooxygenase system from Pseudomonas putida". J. Mol. Biol. 333: 377–392. doi:10.1016/j.jmb.2003.08.028. PMID 14529624.
  6. Sevrioukova, I.F.; Li, H.; Poulos, T.L. (2004). "Crystal structure of putidaredoxin reductase from Pseudomonas putida, the final structural component of the cytochrome P450cam monooxygenase". J. Mol. Biol. 336: 889–902. doi:10.1016/j.jmb.2003.12.067. PMID 15095867.
  7. Smith, N.; Mayhew, M.; Holden, M.J.; Kelly, H.; Robinson, H.; Heroux, A.; Vilker, V.L.; Gallagher, D.T. (2004). "Structure of C73G putidaredoxin from Pseudomonas putida". Acta Crystallogr. D. 60: 816–822. doi:10.1107/s0907444904003348. PMID 15103126.
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