N-acetylglucosaminylphosphatidylinositol deacetylase
| N-acetylglucosaminylphosphatidylinositol deacetylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.5.1.89 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a N-acetylglucosaminylphosphatidylinositol deacetylase (EC 3.5.1.89) is an enzyme that catalyzes the chemical reaction
- 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate
Thus, the two substrates of this enzyme are 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol and H2O, whereas its two products are 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol and acetate.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol acetylhydrolase. Other names in common use include N-acetyl-D-glucosaminylphosphatidylinositol acetylhydrolase, N-acetylglucosaminylphosphatidylinositol de-N-acetylase, GlcNAc-PI de-N-acetylase, GlcNAc-PI deacetylase, and acetylglucosaminylphosphatidylinositol deacetylase. This enzyme participates in 3 metabolic pathways: glycosylphosphatidylinositol(gpi)-anchor, and glycan structures - biosynthesis 2.
References
- Doering TL, Masterson WJ, Englund PT, Hart GW (1989). "Biosynthesis of the glycosyl phosphatidylinositol membrane anchor of the trypanosome variant surface glycoprotein. Origin of the non-acetylated glucosamine". J. Biol. Chem. 264 (19): 11168&ndash, 73. PMID 2525555.
- Nakamura, N; Inoue, N; Watanabe, R; Takahashi, M; Takeda, J; Stevens, VL; Kinoshita, T (1997). "Expression cloning of PIG-L, a candidate N-acetylglucosaminyl-phosphatidylinositol deacetylase". J. Biol. Chem. 272 (25): 15834&ndash, 40. doi:10.1074/jbc.272.25.15834. PMID 9188481.
- Watanabe R, Ohishi K, Maeda Y, Nakamura N, Kinoshita T. "Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis". Biochem. J. 339: 185&ndash, 92. doi:10.1042/0264-6021:3390185. PMC 1220143. PMID 10085243.
- Smit TK; Crossman, A; Borissow, CN; Paterson, MJ; Dix, A; Brimacombe, JS; Ferguson, MA (2001). "Specificity of GlcNAc-PI de-N-acetylase of GPI biosynthesis and synthesis of parasite-specific suicide substrate inhibitors". EMBO J. 20 (13): 3322&ndash, 32. doi:10.1093/emboj/20.13.3322. PMC 125529. PMID 11432820.