MRC-5

MERS Coronavirus Particles found in the lumen of the endoplasmic reticulum in an infected MRC-5 cell

MRC-5 (Medical Research Council cell strain 5) is a diploid human cell culture line composed of fibroblasts derived from lung tissue of a 14 week old aborted caucasian male fetus.[1][2] The cell line was isolated by J.P. Jacobs and colleagues in September 1966 from the 7th population doubling of the original strain, and MRC-5 cells themselves are known to reach senescence in around 45 population doublings. [2] [3] [4]

Applications

MRC-5 cells are used to produce several vaccines including MMR, Varicella and Polio. Infected MRC-5 cells secrete the virus, and can be cultured in large volumes suitable for commercial production.

References

  1. ↑ "MRC-5 (ATCC® CCL-171™)". Retrieved 2016-05-19.
  2. 1 2 "AG05965-D Fibroblast from Skin, Lung". Retrieved 2016-05-19.
  3. ↑ Jacobs, J. P.; Jones, C. M.; Baille, J. P. (1970). "Characteristics of a Human Diploid Cell Designated MRC-5". Nature. 227: 168.
  4. ↑ Jacobs, J. P. (1976). "The Status of Human Diploid Cell Strain MRC-5 as an Approved Substrate for the Production of Viral Vaccines". Journal of Biological Standardization. 4: 97–99.


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