Synechococcus elongatus

In the last decade, several strains of Synechococcus elongatus have been produced in laboratory environments, which ultimately led to the production of Synechococcus elongatus UTEX 2973. S. elongatus UTEX 2973 is a mutant hybrid from UTEX 625.[1]

In 1955, William A. Kratz and Jack Myers described a fast-growing cyanobacterial strain, Anacystis nidulans which was deposited in the University of Texas algae culture collection as Synechococcus leopoliensis UTEX 625[1][2] However, that strain had lost its rapid growth property and was also unable to grow at high temperatures, unlike the original strain. In 2015, Jingjie Yu and colleagues, were able to isolate the mutant strain from a mixed culture of Synechococcus UTEX 625. The mutant strain was deposited to the UTEX algae culture collection, and given a new number, UTEX 2979.[1]

Synechococcus elongatus is rod-shaped with its cells typically greater than 2 µm in length. It typically contains 2–3 thylakoid membrane layers forming evenly spaced concentric rings and its carboxysomes and polyphosphate bodies are located in the central cytoplasmic region (Image 1).[1]

The genome sequence of Synechococcus UTEX 2973 was similar to the cyanobacterium Synechococcus PCC 7942. Even though it was isolated from S. elongatus 625, it is most closely related to S. elongatus PCC 7942 with 99.8% similarity. S. elongatus UTEX 2973 contains a SNP to the gene encoding ATP synthase F₁ subunit α, comparable to the corresponding gene in Synechococcus PCC 7942. This specific SNP causes an amino acid substitution at the 252th position of the protein.[1]

Synechococcus elongatus UTEX 2973 is photoautotrophic and has the shortest doubling time under the conditions at “1.9 hours in a BG11 medium at 41 °C under continuous 500 μmoles photons·m−2·s−1 white light with 3% CO₂”.[3] Its carbon and energy sources are utilizing light to fix CO₂ and produce oxygen. It is typically maintained on BG11 media.

Ungerer and colleagues, 2018, used CRISPR/Cpf1, to perform a mutational analysis of S. elongatus UTEX 2973 by identifying the three genes with SNPs associated with rapid growth, atpA, ppnK, and rpaA. They replaced these fast-growth alleles with the wild-type alleles in the broadly studied cyanobacterium, Synechococcus 7942. This resulted in Synechococcus 7942 reduction in doubling time from 6.8 to 2.3 hours[4]