Protein tandem repeats

An array of protein tandem repeats is defined as several (at least two) adjacent copies having the same or similar sequence motifs. These periodic sequences are generated by internal duplications in both coding and non-coding genomic sequences. Repetitive units of protein tandem repeats are considerably diverse, ranging from the repetition of a single amino acid to domains of 100 or more residues.[1][2]

Schematic representation of tandem repeat sequence.
Common examples of protein tandem repeat structures: the WD40 repeat domain of beta-TrCP (green), leucine-rich repeat domain of TLR2 (red), armadillo repeat domain of beta-catenin (blue), ankyrin repeat domain of ANKRA2 (orange), kelch repeat domain of Keap1 (yellow) and HEAT repeat domain of a PP2A regulatory subunit R1a (magenta).

Distribution in proteomes

Tandem repeats are ubiquitous in proteomes and occur in at least 14% of all proteins.[3] For example, they are present in almost every third human protein and even in every second protein from Plasmodium falciparum or Dictyostelium discoideum.[3][4] Tandem repeats with short repetitive units (especially homorepeats) are more frequent than others.[3]

Structure

Approximately half of the tandem repeat regions has intrinsically disordered conformation being naturally unfolded.[5][6][7] The other half of the regions with the stable 3D structure has a plethora of shapes and functions.[8][9] Depending on the length of the repetitive units, their protein structures can be subdivided into five classes:[8]

  1. crystalline aggregates formed by regions with 1 or 2 residue long repeats, archetypical low complexity regions
  2. fibrous structures stabilized by inter-chain interactions with 3-7 residue repeats
  3. elongated structures with repeats of 5–40 residues dominated by solenoid proteins
  4. closed (not elongated) structures with repeats of 30-60 residue long
  5. beads on a string structures with typical size of repeats over 50 residues, which are already large enough to fold independently into stable domains.

Function

Some well-known examples of proteins with tandem repeats are collagen, which plays a key role in the arrangement of the extracellular matrix; alpha-helical coiled coils having structural and oligomerization functions; leucine-rich repeat proteins, which specifically bind a number of globular proteins by their concave surfaces; and zinc-finger proteins, which regulate the expression of genes by binding DNA.

Annotation methods
Main article: List of protein tandem repeat annotation software

Protein tandem repeats can be either detected from sequence or annotated from structure. Sequence-based annotation is usually made by identifying known sequence motifs (e.g. Tetratricopeptide repeat) or recognizing repetitions in sequence. Structure-based methods instead use available PDB structures to recognize repetitive elements.

References
  1. Heringa J (June 1998). "Detection of internal repeats: how common are they?". Current Opinion in Structural Biology. 8 (3): 338–45. doi:10.1016/s0959-440x(98)80068-7. PMID 9666330.
  2. Andrade MA, Ponting CP, Gibson TJ, Bork P (May 2000). "Homology-based method for identification of protein repeats using statistical significance estimates". Journal of Molecular Biology. 298 (3): 521–37. doi:10.1006/jmbi.2000.3684. PMID 10772867.
  3. Marcotte EM, Pellegrini M, Yeates TO, Eisenberg D (October 1999). "A census of protein repeats". Journal of Molecular Biology. 293 (1): 151–60. doi:10.1006/jmbi.1999.3136. PMID 10512723.
  4. Pellegrini M (2015). "Tandem Repeats in Proteins: Prediction Algorithms and Biological Role". Frontiers in Bioengineering and Biotechnology. 3: 143. doi:10.3389/fbioe.2015.00143. PMC 4585158. PMID 26442257.
  5. Tompa P (September 2003). "Intrinsically unstructured proteins evolve by repeat expansion". BioEssays. 25 (9): 847–55. doi:10.1002/bies.10324. PMID 12938174.
  6. Simon M, Hancock JM (2009). "Tandem and cryptic amino acid repeats accumulate in disordered regions of proteins". Genome Biology. 10 (6): R59. doi:10.1186/gb-2009-10-6-r59. PMC 2718493. PMID 19486509.
  7. Jorda J, Xue B, Uversky VN, Kajava AV (June 2010). "Protein tandem repeats - the more perfect, the less structured" (PDF). The FEBS Journal. 277 (12): 2673–82. doi:10.1111/j.1742-4658.2010.07684.x. PMC 2928880. PMID 20553501.
  8. Kajava AV (September 2012). "Tandem repeats in proteins: from sequence to structure". Journal of Structural Biology. 179 (3): 279–88. doi:10.1016/j.jsb.2011.08.009. PMID 21884799.
  9. Paladin L, Hirsh L, Piovesan D, Andrade-Navarro MA, Kajava AV, Tosatto SC (January 2017). "RepeatsDB 2.0: improved annotation, classification, search and visualization of repeat protein structures". Nucleic Acids Research. 45 (D1): D308–D312. doi:10.1093/nar/gkw1136. PMC 5210593. PMID 27899671.
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.