Leukotriene-E4 20-monooxygenase
In enzymology, a leukotriene-E4 20-monooxygenase (EC 1.14.13.34) is an enzyme that catalyzes the chemical reaction
- (7E,9E,11Z,14Z)-(5S,6R)-6-(cystein-S-yl)-5-hydroxyicosa-7,9,11,14- tetraenoate + NADPH + H+ + O2 20-hydroxyleukotriene E4 + NADP+ + H2O
| leukotriene-E4 20-monooxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.14.13.34 | ||||||||
| CAS number | 111940-51-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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The 5 substrates of this enzyme are (7E,9E,11Z,14Z)-(5S,6R)-6-(cystein-S-yl)-5-hydroxyicosa-7,9,11,14-, tetraenoate, NADPH, H+, and O2, whereas its 3 products are 20-hydroxyleukotriene E4, NADP+, and H2O.
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is (7E,9E,11Z,14Z)-(5S,6R)-6-(cystein-S-yl)-5-hydroxyicosa-7,9,11,14-te traenoate,NADPH:oxygen oxidoreductase (20-hydroxylating). This enzyme is also called leukotriene-E4 omega-hydroxylase. This enzyme participates in arachidonic acid metabolism.
References
- Orning L (December 1987). "Omega-oxidation of cysteine-containing leukotrienes by rat-liver microsomes. Isolation and characterization of omega-hydroxy and omega-carboxy metabolites of leukotriene E4 and N-acetylleukotriene E4". European Journal of Biochemistry. 170 (1–2): 77–85. doi:10.1111/j.1432-1033.1987.tb13669.x. PMID 2826163.