Ginkgo Bioworks

Ginkgo Bioworks is an American biotech company founded in 2009 by scientists from MIT and headed by Tom Knight. The company specializes in using genetic engineering to produce bacteria with industrial applications.[1][2][3][4] Ginkgo Bioworks is an analytics company that designs organisms for customers in a range of industries. It is the self-proclaimed "Organism Company" and is one of the world's largest privately held biotech companies.[5] As of 2019, it was valued at $4.2 billion.[6]

Ginkgo Bioworks
IndustryBiotechnology
Founded2009 (2009) in Boston, Massachusetts, United States of America
FoundersTom Knight, Jason Kelly, Reshma Shetty, Barry Canton, Austin Che
Headquarters,
United States of America
Key people
Tom Knight
Jason Kelly
Reshma Shetty
Barry Canton
Austin Che
Christina Agapakis, Creative Director
Number of employees
~200
Websiteginkgobioworks.com

Ginkgo Bioworks designs microbes in analytical labs it calls synthetic biology foundries, which comprise software and hardware tools that allow for rapid prototyping and high-throughput screening. Several publicly-stated products include chemical intermediates, flavors, and fragrances; as well as microbes that provide health benefits to the human gut microbiome and agricultural crop root microbiomes.

Products and Services

Ginkgo Bioworks specializes in creating tools for improving cultured ingredients, high-throughput strain improvement, and enzyme design.[7] Cultured ingredients are made by yeast fermentation, and the yeast can be made to produce compounds for use as flavors, fragrances, food ingredients, cosmetics, and more.[8]

High-throughput strain improvement is the next step after figuring out how to make cultured ingredients. Strain improvement seeks to increase efficiency, reduces cost, and improve the sustainability of products made by custom organisms. Enzyme design looks for ways to either use existing enzymes for other purposes or looks for ways to make enzymes do their job better. Enzymes act as catalysts that speed up biochemical reactions. Examples of the industrial use of enzymes include cheesemaking, pharmaceutical production, and clothing manufacturing.

In 2019, Ginkgo Bioworks announced that it had produced a print magazine, called Grow.[9][10][11] The company is giving the magazines away for free.[11]

Synthetic Biology Foundries

BioworksS1

BioworksS1 was the world's first organism foundry capable of complete automation of organism strain engineering. The foundry can perform up to 15,000 automated lab tasks per month allowing genetic engineers at Ginkgo Bioworks to test thousands of new organism designs every month without the need to perform the majority of repetitive lab work.[12]

BioworksS2

BioworksS2 is an upgraded version of BIOWORKS1 that is twice as big. It provides a 6X productivity improvement on the original BioworksS1 foundry through more efficient integration and miniaturization of equipment.[13]

Funding

In its early years, the company was supported primarily through federal research grants. Working on a variety of projects with different government agencies, such as the National Science Foundation and Defense Advanced Research Projects Agency (DARPA), before gaining access to venture capital funding in the summer of 2014.

Government awards/grants

Award/Grant Funding Agency Value Year
Adapting wireless glucometer to boost opioid dependence medication compliance Department of Health and Human Services $150,000 2013
Controlling Antibiotic Resistance by Vaccinating Bacterial Populations Defense Advanced Research Projects Agency (DARPA), Department of Defense $150,000 2012
Controlling Antibiotic Resistance by Vaccinating Bacterial Populations Defense Advanced Research Projects Agency (DARPA), Department of Defense $1,750,000 2014
SBIR Phase 1:Volatile gene expression reporters for use during fermentation National Science Foundation $99,981 2009
SBIR Phase I: Bioproduction of Feedstock Amino Acids National Science Foundation $150,000 2013
SBIR Phase I: Creating Plant Inspired Fragrances Via Fermentation National Science Foundation $150,000 2015
SBIR Phase I: Novel proteolysis-based tools for metabolic engineering of amino acid producing strains National Science Foundation $150,000 2011
SBIR Phase II: Novel Proteolysis-based Tools for Metabolic Engineering National Science Foundation $499,971 2013

Seed

On July 16, 2014 Ginkgo Bioworks received $120K in seed funding from Y Combinator, TSVC, CRCM ventures, 11.2 Capital, Farzad Nazem, Todd Corenson, and David Spector.[14]

Series A

On March 19, 2015 Ginkgo Bioworks received $9 million dollars in funding from iGlobe Partners, Vast Ventures, OS Fund,[15] Felicis Ventures, Data Collective, Ian McNish, and David Beyer.[16]

Series B

Ginkgo Bioworks had a pre-money valuation of $250 million and raised $45 million on July 23, 2015 during their series B funding. Investors include: Viking Global Investors, OS Fund, Felicis Ventures, and 11.2 Capital.[17]

Series C

Ginkgo Bioworks had pre-money evaluation of $350 million before receiving $100 million in its series C funding round on June 8, 2016. The funding came from a number of old and new investors including: Y Combinator, Viking Global Investors, Senator Investment Group, Baillie Gifford, and Allen & Company.[18]

Series D

On December 14, 2017 Ginkgo Bioworks completed its series D funding round. The company had a pre-money valuation of $725 million and gained an additional $275 million dollars in venture capital. The $275 million came from Viking Global Investors, Y Combinator's Continuity Fund, Cascade Investment, and Bill Gates. Ginkgo Bioworks is using the series D funding to build a BioworksS3 at its headquarters in Boston and hire additional employees.[19]

Further Investments

In 2019, Ginkgo Bioworks received $290 million in September and a $350 million fund in October. [5]

Partnerships

Amyris

On June 29, 2016 Ginkgo Bioworks and Amyris entered into a collaboration agreement and announced their intention to partner to commercialize up to 70 cultured ingredients.[20] The partnership was entered into in November 2017. Ginkgo Bioworks agreed to pay up to $20 million in technology access fees to Amyris. It paid $15 million upfront in 2016, but disputes resulted in the final $5 million never being paid. The pair continue to collaborate on "limited research and development", although Amyris hasn't recorded revenue from Gingko Bioworks since the initial upfront payment in 2016.

All of the founders of Ginkgo Bioworks created an affiliate, Stegodon Corporation, to provide and assume certain debt notes to Amyris while keeping the debt off Ginkgo Bioworks' books. Amyris' failure to pay debts at certain maturity dates has resulted in Stegodon Corporation receiving some patents from the company as collateral, several of which it has paid to upkeep with the U.S. Patent and Trademark Office, proving that Stegodon Corporation is actively managed.[21] Ginkgo Bioworks hasn't commented publicly on the existence of Stegodon Corporation or the affiliate's purpose. On June 29, 2018, Amyris secured a $35 million loan to repay all amounts owed to Stegodon Corporation.[22]

Archer Daniels Midland Company (ADM)

On September 23, 2016, Ginkgo Bioworks announced a partnership with Archer Daniels Midland Company (ADM) to design organisms capable of producing cultured ingredients for use in agricultural processing and food ingredient industries.[23] ADM hopes to help Ginkgo Bioworks create new organisms that reduce cost and increase the sustainability of their agricultural processing and food ingredient business. ADM plans on using Ginkgo Bioworks for solving problems faced by its customers through providing novel ingredient production and formulation processes.

Bayer

On March 20, 2018, Ginkgo Bioworks announced it had finalized a previously-announced joint venture with the agriscience arm of Bayer, which will be called Joyn Bio.[24] The joint venture will focus on designing soil microbes to improve the root system microbiomes of agricultural crops, specifically providing nitrogen fixation to reduce the need for synthetic fertilizer applications. Joyn Bio is similar to the $600 million joint venture between Monsanto and Novozymes called the BioAg Alliance, which commercialized its first products in December 2016 after conducting years of field testing.[25] Monsanto is now a part of Bayer.

Cargill

On September 22, 2016, Ginkgo Bioworks announced a partnership with Cargill to improve industrial fermentation processes.[26]

Genomatica

On September 29, 2016, Ginko Bioworks announced a partnership with Genomatica.[27] The companies will be focusing on scaling and commercializing the intermediate and speciality chemical production of organisms made by Ginkgo Bioworks. Genomatica designs and improves industrial process of bio-manufacturing by creating economic and technical solutions to problems faced within the bio-manufacturing industry. The goal of this partnership is for both companies to work together create a unified offer for the market that provides organism design and high volume manufacturing capacity.[28]

Prospect Bio

On October 19, 2016, Ginkgo Bioworks announced a partnership with Prospect Bio to create a new biosensor to improve the speed and lower the cost of organism prototyping for new strain development. The biosensor under development will make the process of organism screening (testing for a desired trait) faster. Organism screening is one of the slowest processes of the design, build, test system Ginkgo Bioworks uses for organism development. Normally, each organism created by Ginkgo Bioworks has to be screened individually. A biosensor allows for the screening of many organisms at one time. Ginkgo Bioworks is hoping for a forty-fold decrease in the cost of screening and increase the speed of creating commercial ready organisms.[29]

Synlogic

On December 8, 2017, Ginkgo Bioworks and Synlogic announced a collaboration to engineer microbes for probiotic and gut-microbiome-based medicines.

Mergers/acquisitions

Gen9

On January 20, 2017 Ginkgo Bioworks announced that it had acquired Gen9[30] — a company specializing in DNA synthesis. Ginkgo Bioworks previously contracted Gen9 to deliver 300 million base pairs in 2017,[31] making Ginkgo Bioworks Gen9's biggest customer prior to the acquisition. After the acquisition, only 10 Gen9 employees remained on staff with Ginkgo Bioworks.[31]

References

  1. Molteni, Megan (14 September 2017). "With Designer Bacteria, Crops One Day Could Fertilize Themselves". Wired. Retrieved 4 December 2017.
  2. Herper, Mathew (8 June 2016). "Boston Startup Raises $100 Million To Use Synthetic Biology To Design Microbes For Industry". Forbes. Retrieved 4 December 2017.
  3. Adams, Susan (14 September 2017). "Bayer And Ginkgo Bioworks, A Startup, Aim To Make Crops Produce Their Own Nitrogen Fertilizer". Forbes. Retrieved 4 December 2017.
  4. Weisman, Robert (29 September 2016). "Ginkgo Bioworks opens production site for custom cells". Boston Globe. Retrieved 4 December 2017.
  5. "Ginkgo Bioworks raises $350 million fund for biotech spinouts". Reuters. 2019-10-09. Retrieved 2019-11-07.
  6. "Ginkgo Bioworks CEO Wants Biology to Manufacture Physical Goods". Bloomberg. Retrieved 2019-11-07.
  7. "About ginkgobioworks".
  8. Botstein, D; Chervitz, SA; Cherry, JM (August 1997). "Ginko". Science. 277: 1259–60. doi:10.1126/science.277.5330.1259. PMC 3039837. PMID 9297238.
  9. Jen, Ginkgo | (2019-10-01). "So incredibly proud and excited to debut the first issue of Ginkgo's annual magazine, Grow. Synthetic biology is beautiful, complex, messy, and full of what if's. We wanted to create a brand that showed people that.pic.twitter.com/HPFDSguHfo". @ginkgo. Retrieved 2019-11-07.
  10. Jen, Ginkgo | (2019-10-03). "sign up here for updates on how to get a copy!!!". @Ginkgo. Retrieved 2019-11-07.
  11. "Grow by Ginkgo | A Magazine About Synthetic Biology". Grow by Ginkgo. Retrieved 2019-12-12.
  12. "Ginkgo Bioworks Opens Its Genetic Engineering Foundry".
  13. "Boston-based Ginkgo BioWorks Opens World's Largest Biotech Foundry to Increase Production Capacity".
  14. "Seed - Ginkgo Bioworks".
  15. "Series A - Ginkgo Bioworks".
  16. "Ginkgo Bioworks Secures $9 Million in a Series A Financing - SynBioBeta".
  17. "Ginkgo Bioworks Closes $45M Series B - SynBioBeta".
  18. "Ginkgo Bioworks Secures $100 Million Series C Investment".
  19. "Ginkgo Bioworks secures $275 million in Series D, valuing the company at over $1 billion". TechCrunch.
  20. "Ginkgo Bioworks and Amyris Partner to Accelerate Commercialization of Bio-Based Products". Amyris.
  21. "SEC Filing, 10-K for the Year Ended December 31, 2017. pp. 31".
  22. "Amyris 8-K Filing 07/02/2018".
  23. "Ginkgo Bioworks to Design Custom Microorganisms for ADM. prnewswire".
  24. "Bayer, Ginkgo Bioworks names new tech partnership Joyn Bio". AGDAILY.
  25. "The BioAg Alliance launches new yield-boosting microbial seed coating".
  26. Manning, Lauren (September 23, 2016). "Ag Industry Brief: Monsanto Licenses CRISPR, Ginkgo Bioworks Partners with Cargill, California Wants to Regulate Cow Farts". AgFunderNews.
  27. "Ginkgo Bioworks and Genomatica Forge Alliance to Accelerate Commercialization of More Sustainable High-Volume Chemicals". GlobeNewswire News Room.
  28. Lane, Jim (October 19, 2016). "The Rise of Organic Manufacturing: Ginkgo, Amyris, Genomatica's circle of innovation is a trend to watch". Biofuels Digest.
  29. Stevenson, Christine (October 2016). "Ginkgo Bioworks and Prospect Bio will Collaborate to Develop and Deploy Biosensors". SynBioBeta.
  30. "Organism Company Ginkgo Bioworks Acquires Synthetic DNA Supplier Gen9". SynBioBeta. January 23, 2017.
  31. "How a biotech startup rose, and then fell - The Boston Globe". BostonGlobe.com. Retrieved 2019-11-07.


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