Germacrene A alcohol dehydrogenase

Germacrene A alcohol dehydrogenase (EC 1.1.1.314) is an enzyme with systematic name germacra-1(10),4,11(13)-trien-12-ol:NADP+ oxidoreductase.[1][2] This enzyme catalyses the following chemical reaction

germacra-1(10),4,11(13)-trien-12-ol + 2 NADP+ + H2O germacra-1(10),4,11(13)-trien-12-oate + 2 NADPH + 3 H+ (overall reaction)
(1a) germacra-1(10),4,11(13)-trien-12-ol + NADP+ germacra-1(10),4,11(13)-trien-12-al + NADPH + H+
(1b) germacra-1(10),4,11(13)-trien-12-al + NADP+ + H2O germacra-1(10),4,11(13)-trien-12-oate + NADPH + 2 H+
Germacrene A alcohol dehydrogenase
Identifiers
EC number1.1.1.314
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

In Lactuca sativa this enzyme is a mutifunctional enzyme with EC 1.14.13.123.

References
  1. de Kraker JW, Franssen MC, Dalm MC, de Groot A, Bouwmeester HJ (April 2001). "Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene a hydroxylase and NADP+-dependent sesquiterpenoid dehydrogenase(s) involved in sesquiterpene lactone biosynthesis". Plant Physiology. 125 (4): 1930–40. doi:10.1104/pp.125.4.1930. PMC 88848. PMID 11299372.
  2. Nguyen DT, Göpfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK (May 2010). "Biochemical conservation and evolution of germacrene A oxidase in asteraceae". The Journal of Biological Chemistry. 285 (22): 16588–98. doi:10.1074/jbc.M110.111757. PMC 2878029. PMID 20351109.
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