Galactoside acetyltransferase

Galactoside acetyltransferase (also known as Galactoside O-acetyltransferase, thiogalactoside transacetylase and GAT) is an enzyme that transfers an acetyl group from acetyl-CoA to galactosides, glucosides and lactosides. It is coded for by the lacA gene of the lac operon in E. coli.[1]

GAT in complex with CoA and two molecules/active site of IPTG viewed perpendicular to the molecular threefold axis of the enzyme
Galactoside O-acetyltransferase
Identifiers
EC number2.3.1.18
CAS number9029-94-1
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Thiogalactoside acetyltransferase
Identifiers
OrganismEscherichia coli
SymbollacA
Entrez945674
RefSeq (Prot)NP_414876
UniProtP07464

Reaction

It catalyzes the following reaction:

acetyl-CoA + beta-D-galactoside → CoA + 6-acetyl-beta-D-galactoside

The kinetics of the enzyme were delineated in 1995.[2]

Biological role

The enzyme's role in the classical E.coli lac operon remains unclear.[1][3] However, the enzyme's cellular role may be to detoxify non-metabolizable pyranosides by acetylating them and preventing their reentry into the cell.[1][4]

See also

References
  1. Wang XG, Olsen LR, Roderick SL (April 2002). "Structure of the lac operon galactoside acetyltransferase". Structure. 10 (4): 581–8. doi:10.1016/S0969-2126(02)00741-4. PMID 11937062.
  2. Lewendon A, Ellis J, Shaw WV (November 1995). "Structural and mechanistic studies of galactoside acetyltransferase, the Escherichia coli LacA gene product". The Journal of Biological Chemistry. 270 (44): 26326–31. doi:10.1074/jbc.270.44.26326. PMID 7592843.
  3. Roderick SL (June 2005). "The lac operon galactoside acetyltransferase". Comptes Rendus Biologies. 328 (6): 568–75. doi:10.1016/j.crvi.2005.03.005. PMID 15950163.
  4. "Thiogalactoside transacetylase of the lactose operon as an enzyme for detoxification". 1976. Cite journal requires |journal= (help)


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