Aspartate dehydrogenase
Aspartate dehydrogenase (EC 1.4.1.21) is an enzyme that catalyzes the chemical reaction
- L-aspartate + H2O + NAD(P)+ oxaloacetate + NH3 + NAD(P)H + H+
| Aspartate dehydrogenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.4.1.21 | ||||||||
| CAS number | 37278-97-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
The 4 substrates of this enzyme are L-aspartate, water, nicotinamide adenine dinucleotide ion, and nicotinamide adenine dinucleotide phosphate ion, whereas its 5 products are oxaloacetate, ammonia, NADH, nicotinamide adenine dinucleotide phosphate, and hydrogen ion.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is L-aspartate:NAD(P)+ oxidoreductase (deaminating). Other names in common use include NAD-dependent aspartate dehydrogenase, NADH2-dependent aspartate dehydrogenase, and NADP+-dependent aspartate dehydrogenase. This enzyme participates in nicotinate and nicotinamide metabolism.
Structural studies
As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 2DC1.
References
- Tong L; Savchenko, A; Yakunin, A; Zhang, R; Edwards, A; Arrowsmith, C; Tong, L (2003). "Aspartate dehydrogenase, a novel enzyme identified from structural and functional studies of TM1643". J. Biol. Chem. 278 (10): 8804–8. doi:10.1074/jbc.M211892200. PMID 12496312.
- Okamura T, Noda H, Fukuda S, Ohsugi M (August 1998). "Aspartate dehydrogenase in vitamin B12-producing Klebsiella pneumoniae IFO 13541". J. Nutr. Sci. Vitaminol. Tokyo. 44 (4): 483–90. doi:10.3177/jnsv.44.483. PMID 9819709.
- Kazakova OW; Kariakina, T. I.; Weinova, M. K.; Sidelnikova, L. I.; Kazakova, O. W. (1981). "The synthesis of aspartic acid in Rhizobium lupini bacteroids". Plant Soil. 61 (1–2): 145–156. doi:10.1007/BF02277371.