Acetyl-CoA C-acetyltransferase

In enzymology, an acetyl-CoA C-acetyltransferase (EC 2.3.1.9) is an enzyme that catalyzes the chemical reaction

2 acetyl-CoA CoA + acetoacetyl-CoA
acetyl-CoA C-acetyltransferase
Crystallographic structure of the tetrameric acetoacetyl-CoA thiolase (EC:2.3.1.9) from the Gram-negative bacterium Z. ramigera.[1]
Identifiers
EC number2.3.1.9
CAS number9027-46-7
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

Hence, this enzyme has one substrate, acetyl-CoA, and two products, CoA and acetoacetyl-CoA.

Acetyl-CoA C-acetyltransferase belongs to the thiolase family of enzymes.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:acetyl-CoA C-acetyltransferase. Other names in common use include acetoacetyl-CoA thiolase, beta-acetoacetyl coenzyme A thiolase, 2-methylacetoacetyl-CoA thiolase [misleading], 3-oxothiolase, acetyl coenzyme A thiolase, acetyl-CoA acetyltransferase, acetyl-CoA:N-acetyltransferase, and thiolase II. This enzyme participates in 10 metabolic pathways: fatty acid metabolism, synthesis and degradation of ketone bodies, valine, leucine and isoleucine degradation, lysine degradation, tryptophan metabolism, pyruvate metabolism, benzoate degradation via coa ligation, propanoate metabolism, butanoate metabolism, and two-component system - general.

Isozymes

Human genes encoding acetyl-CoA C-acetyltransferases include:

acetyl-Coenzyme A acetyltransferase 1 (acetoacetyl Coenzyme A Thiolase)
Identifiers
SymbolACAT1
NCBI gene38
HGNC93
OMIM607809
RefSeqNM_000019
UniProtP24752
Other data
EC number2.3.1.9
LocusChr. 11 q22.3-23.1
acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase)
Identifiers
SymbolACAT2
NCBI gene39
HGNC94
OMIM100678
RefSeqNM_005891
UniProtQ9BWD1
Other data
EC number2.3.1.9
LocusChr. 6 q25.3-q26

References

  1. PDB: 2WKT; Meriläinen G, Poikela V, Kursula P, Wierenga RK (November 2009). "The thiolase reaction mechanism: the importance of Asn316 and His348 for stabilizing the enolate intermediate of the Claisen condensation". Biochemistry. 48 (46): 11011–25. doi:10.1021/bi901069h. PMID 19842716.

Further reading

  • LYNEN F, OCHOA S (1953). "Enzymes of fatty acid metabolism". Biochim. Biophys. Acta. 12 (1–2): 299–314. doi:10.1016/0006-3002(53)90149-8. PMID 13115439.
  • Stern JR, Drummond GI, Coon MJ, del Campillo A (1960). "Enzymes of ketone body metabolism. I. Purification of an acetoacetate-synthesizing enzyme from ox liver". J. Biol. Chem. 235: 313–317. PMID 13834445.


This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.