Seminaphtharhodafluor

Seminaphtharhodafluor
Names
Other names
Seminaphtharhodafluorescein; Seminaphthorhodafluor
Identifiers
3D model (JSmol)
ChemSpider
Properties
C24H15NO4
Molar mass 381.39 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Seminaphtharhodafluor or SNARF is a fluorescent dye that changes color with pH. It can be used to construct optical biosensors that use enzymes that change pH.

The absorption peak of the derivative carboxy-SNARF at pH 6.0 is at wavelength (515 and) 550 nm, while that at pH 9.0 is at 575 nm.

The emission peak of carboxy-SNARF at pH 6.0 is at wavelength 585 nm, while that at pH 9.0 is at 640 nm.[1]

SNARF-1 can serve as a substrate for the MRP1 (multidrug resistance-associated protein-1) drug transporter, to measure the activity of the MRP1 transporter. For this purpose, an acetomethoxyester group is added to SNARF-1. Cellular esterases cleave off SNARF-1, and its transport out of the cells can be measured by following the loss of fluorescence from the cells.[2]

References

  1. http://www.lifetechnologies.com/order/catalog/product/C1270
  2. Weekes-MP et al., Science Vol 340, April 2013


This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.