Mini-ykkC RNA motif

mini-ykkC RNA motif
	Predicted secondary structure and sequence conservation of mini-ykkC
Identifiers
Symbol	mini-ykkC
Rfam	RF01068
Other data
RNA type	Cis-reg
Domain(s)	Bacteria
SO	0005836
PDB structures	PDBe

The mini-ykkC RNA motif (later renamed Guanidine-II riboswitch) was discovered as a putative RNA structure that is conserved in bacteria.^[1] The motif consists of two conserved stem-loops whose terminal loops contain the RNA sequence ACGR, where R represents either A or G. Mini-ykkC RNAs are widespread in Proteobacteria, but some are predicted in other phyla of bacteria. It was expected that the RNAs are cis-regulatory elements, because they are typically located upstream of protein-coding genes.

The genes that are apparently controlled by mini-ykkC RNAs bear a resemblance to the genes controlled by ykkC-yxkD leader (guanidine-I), and nine gene families are common to both. Therefore, it was proposed that these two RNA classes have the same function. The complex structure and many conserved nucleotides found in the ykkC-yxkD leader are not present in the mini-ykkC RNA motif. Despite this, it was shown that each of the mini-ykkC two stem-loop structures directly binds free guanidine. Therefore, mini-ykkC RNA motif represents a distinct class of guanidine-sensing RNAs called Guanidine-II riboswitch.^[2] Its crystal structure was also determined.^[3]

References

1 2 Weinberg Z, Barrick JE, Yao Z, et al. (2007). "Identification of 22 candidate structured RNAs in bacteria using the CMfinder comparative genomics pipeline". Nucleic Acids Res. 35 (14): 4809–19. doi:10.1093/nar/gkm487. PMC 1950547. PMID 17621584.
↑ Sherlock, Madeline E.; Malkowski, Sarah N.; Breaker, Ronald R. (2017-01-17). "Biochemical Validation of a Second Guanidine Riboswitch Class in Bacteria". Biochemistry. 56 (2): 352–358. doi:10.1021/acs.biochem.6b01270. ISSN 1520-4995. PMC 5340285. PMID 28001368.
↑ Huang, Lin; Wang, Jia; Lilley, David M. J. (2017-06-22). "The Structure of the Guanidine-II Riboswitch". Cell Chemical Biology. 24 (6): 695–702.e2. doi:10.1016/j.chembiol.2017.05.014. ISSN 2451-9456. PMC 5486947. PMID 28529131.

External links

Page for Mini-ykkC_RNA_motif at Rfam

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[Weinberg2007-1] 1 2 Weinberg Z, Barrick JE, Yao Z, et al. (2007). "Identification of 22 candidate structured RNAs in bacteria using the CMfinder comparative genomics pipeline". Nucleic Acids Res. 35 (14): 4809–19. doi:10.1093/nar/gkm487. PMC 1950547. PMID 17621584.

[2] Sherlock, Madeline E.; Malkowski, Sarah N.; Breaker, Ronald R. (2017-01-17). "Biochemical Validation of a Second Guanidine Riboswitch Class in Bacteria". Biochemistry. 56 (2): 352–358. doi:10.1021/acs.biochem.6b01270. ISSN 1520-4995. PMC 5340285. PMID 28001368.

[3] Huang, Lin; Wang, Jia; Lilley, David M. J. (2017-06-22). "The Structure of the Guanidine-II Riboswitch". Cell Chemical Biology. 24 (6): 695–702.e2. doi:10.1016/j.chembiol.2017.05.014. ISSN 2451-9456. PMC 5486947. PMID 28529131.