Kinetic capillary electrophoresis

Kinetic capillary electrophoresis or KCE is capillary electrophoresis of molecules that interact during electrophoresis. KCE was introduced and developed by Professor Sergey Krylov and his research group at York University, Toronto, Canada.^[1] It serves as a conceptual platform for development of homogeneous chemical affinity methods for studies of molecular interactions (measurements of binding and rate constants) and affinity purification (purification of known molecules and search for unknown molecules). Different KCE methods are designed by varying initial and boundary conditions – the way interacting molecules enter and exit the capillary. Several KCE methods were described: non-equilibrium capillary electrophoresis of the equilibrium mixtures (NECEEM),^[2] sweeping capillary electrophoresis (SweepCE),^[3] plug-plug KCE (ppKCE).^[4] More detailed description and several applications of KCE methods (measuring equilibrium and rate constants of molecular interactions, quantitative affinity analysis of proteins, thermochemistry of protein–ligand interactions, selection of aptamers, determination of temperature inside a capillary) can be found in a PDF presentation: KCE ia a conceptual platform for kinetic homogeneous affinity methods.

References

↑ Petrov A, Okhonin V, Berezovski M, Krylov SN (Dec 2005). "Kinetic capillary electrophoresis (KCE): a conceptual platform for kinetic homogeneous affinity methods". J Am Chem Soc. 127 (48): 17104–10. doi:10.1021/ja056232l. PMID 16316258.
↑ Berezovski M, Krylov SN (Nov 2002). "Nonequilibrium capillary electrophoresis of equilibrium mixtures—a single experiment reveals equilibrium and kinetic parameters of protein–DNA interactions". J Am Chem Soc. 124 (46): 13674–75. doi:10.1021/ja028212e. PMID 12431087.
↑ Okhonin V, Berezovski M, Krylov SN (Jun 2004). "Sweeping capillary electrophoresis: a non-stopped-flow method for measuring bimolecular rate constant of complex formation between protein and DNA". J Am Chem Soc. 126 (23): 7166–67. doi:10.1021/ja0481124. PMID 15186140.
↑ Okhonin V, Petrov AP, Berezovski M, Krylov SN (Jul 2006). "Plug-plug kinetic capillary electrophoresis: method for direct determination of rate constants of complex formation and dissociation". Anal. Chem. 78 (14): 4803–10. doi:10.1021/ac060108i. PMID 16841898.

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[1] Petrov A, Okhonin V, Berezovski M, Krylov SN (Dec 2005). "Kinetic capillary electrophoresis (KCE): a conceptual platform for kinetic homogeneous affinity methods". J Am Chem Soc. 127 (48): 17104–10. doi:10.1021/ja056232l. PMID 16316258.

[2] Berezovski M, Krylov SN (Nov 2002). "Nonequilibrium capillary electrophoresis of equilibrium mixtures—a single experiment reveals equilibrium and kinetic parameters of protein–DNA interactions". J Am Chem Soc. 124 (46): 13674–75. doi:10.1021/ja028212e. PMID 12431087.

[3] Okhonin V, Berezovski M, Krylov SN (Jun 2004). "Sweeping capillary electrophoresis: a non-stopped-flow method for measuring bimolecular rate constant of complex formation between protein and DNA". J Am Chem Soc. 126 (23): 7166–67. doi:10.1021/ja0481124. PMID 15186140.

[4] Okhonin V, Petrov AP, Berezovski M, Krylov SN (Jul 2006). "Plug-plug kinetic capillary electrophoresis: method for direct determination of rate constants of complex formation and dissociation". Anal. Chem. 78 (14): 4803–10. doi:10.1021/ac060108i. PMID 16841898.