Immunoscreening

Immunoscreening is a method of biotechnology to detect a polypeptide produced from a cloned gene.

Clones are screened for the presence of the gene product (a protein). This strategy requires first that a gene library is implemented in an expression vector, and that antiserum to the protein is available. Radioactivity or an enzyme is coupled generally with the secondary antibody. The radioactivity/enzyme linked secondary antibody can be purchased commercially and can detect different antigens. In commercial diagnostics labs, labelled primary antibodies are also used.[1]

See also

References

  1. ↑ Karam, James (November 26, 1990). Methods in Nucleic Acids Research. CRC Press. p. 309. ISBN 0849353114.


This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.