Holo-(acyl-carrier-protein) synthase
| phosphopantetheinyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.7.8.7 | ||||||||
| CAS number | 37278-30-1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a holo-[acyl-carrier-protein] synthase (EC 2.7.8.7) is an enzyme that catalyzes the chemical reaction:
CoA-[4'-phosphopantetheine] + apo-acyl carrier protein adenosine 3',5'-bisphosphate + holo-acyl carrier protein
This enzyme belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. The systematic name of this enzyme class is CoA-[4'-phosphopantetheine]:apo-[acyl-carrier-protein] 4'-pantetheinephosphotransferase. Other names in common use include acyl carrier protein holoprotein (holo-ACP) synthetase, holo-ACP synthetase, coenzyme A:fatty acid synthetase apoenzyme 4'-phosphopantetheine, transferase, holosynthase, acyl carrier protein synthetase, holo-ACP synthase, PPTase, AcpS, ACPS, acyl carrier protein synthase, P-pant transferase, and CoA:apo-[acyl-carrier-protein] pantetheinephosphotransferase. This enzyme participates in pantothenate and coa biosynthesis.
Structural studies
As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes 1F7L, 1F7T, 1F80, 1FTE, 1FTF, 1FTH, 2JBZ, and 2JCA.
References
- Elovson J, Vagelos PR (1968). "Acyl carrier protein. X. Acyl carrier protein synthetase". J. Biol. Chem. 243 (13): 3603&ndash, 11. PMID 4872726.
- Prescott DJ, Vagelos PR (1972). "Acyl carrier protein". Adv. Enzymol. Relat. Areas Mol. Biol. 36: 269&ndash, 311. doi:10.1002/9780470122815.ch8. PMID 4561013.
- Reid R, Khosla C, Walsh CT (1996). "A new enzyme superfamily - the phosphopantetheinyl transferases". Chem. Biol. 3 (11): 923&ndash, 36. doi:10.1016/S1074-5521(96)90181-7. PMID 8939709.
- Walsh CT, Gehring AM, Weinreb PH, Quadri LE, Flugel RS (1997). "Post-translational modification of polyketide and nonribosomal peptide synthases". Curr. Opin. Chem. Biol. 1 (3): 309&ndash, 15. doi:10.1016/S1367-5931(97)80067-1. PMID 9667867.
- Mootz HD, Finking R, Marahiel MA (2001). "4'-phosphopantetheine transfer in primary and secondary metabolism of Bacillus subtilis". J. Biol. Chem. 276 (40): 37289&ndash, 98. doi:10.1074/jbc.M103556200. PMID 11489886.
- Joshi AK, Zhang L, Rangan VS, Smith S (2003). "Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity". J. Biol. Chem. 278 (35): 33142&ndash, 9. doi:10.1074/jbc.M305459200. PMID 12815048.