High Five cells

High Five (officially called BTI-Tn-5B1-4) is an insect cell line that originated from the ovarian cells of the cabbage looper, Trichoplusia ni.[1] It was developed by the Boyce Thompson Institute for Plant Research, Ithaca, NY.

High Five cells have become one of the most commonly used cell lines for recombinant protein expression using baculovirus or transfection, and have been demonstrated, in a number of cases, to express more recombinant protein than other lepidopteran cell lines, such as Sf9 cells.[2][3][4] They can be grown in the absence of serum, and can be cultured in a loose attached state or in suspension.[5] Hi5 cells produce abundant microRNAs (miRNAs), small interfering RNAs (siRNAs), and PIWI-interacting RNAs (piRNAs), making it suitable to study all three types of small silencing RNAs.[6]

References

  1. ↑ Wickham TJ, Davis T, Granados RR, Shuler ML, Wood HA (1992). "Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system". Biotechnol Prog. 8 (5): 391–396. doi:10.1021/bp00017a003. PMID 1369220.
  2. ↑ Davis TR, Trotter KM, Granados RR, Wood HA (1992). "Baculovirus expression of alkaline phosphatase as a reporter gene for evaluation of production, glycosylation and secretion". Bio/Technology. 10 (10): 1148–1150. doi:10.1038/nbt1092-1148. PMID 1368794.
  3. ↑ Wickham TJ, Nemerow GR (1993). "Optimization of growth methods and recombinant protein production in BTI-Tn-5B1-4 insect cells using the baculovirus expression system". Biotechnol Prog. 9 (1): 25–30. doi:10.1021/bp00019a004. PMID 7764044.
  4. ↑ Granados RR, Guoxun L, Derksen AC, McKenna KA (1994). "A New Insect Cell Line from Trichoplusia ni (BTI-Tn-5B1-4) Susceptible to Trichoplusia ni Single Enveloped Nuclear Polyhedrosis". Virus. J. Invertebr. Pathol. 64: 260–266. doi:10.1016/s0022-2011(94)90400-6.
  5. ↑ Invitrogen (31 March 2015). "Cell Lines". Growth and Maintenance of Insect cell lines (PDF). A.0. Thermo Fisher Scientific. Retrieved 2015-11-09.
  6. ↑ Fu, Yu; Yang, Yujing; Zhang, Han; Farley, Gwen; Wang, Junling; Quarles, Kaycee A; Weng, Zhiping; Zamore, Phillip D (2018-01-29). "The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology". eLife. 7. doi:10.7554/eLife.31628. ISSN 2050-084X.
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