HIS3
Imidazoleglycerol-phosphate dehydratase | |
---|---|
Identifiers | |
Organism | |
Symbol | HIS3 |
Entrez | 854377 |
HomoloGene | 6979 |
RefSeq (mRNA) | NM_001183621.1 |
RefSeq (Prot) | NP_014845.1 |
UniProt | P06633 |
Other data | |
EC number | 4.2.1.19 |
Chromosome | XV: 0.72 - 0.72 Mb |
The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis.[1] It is analogous to hisB in Escherichia coli.
Exploits
Mutations in Escherichia coli's analogous gene, hisB allows researchers to select only those individuals expressing the HIS3 gene included on a plasmid. The HIS3 gene is coupled to a certain promoter which can only be activated by successful binding of the relevant transcription factors. This is used in certain methods of bacterial two-hybrid screening to allow the survival of E. coli in which a desired protein-DNA or protein-protein interaction is taking place.[2]
References
- ↑ HIS3/YOR202W Summary
- ↑ Joung JK, Ramm EI, Pabo CO (June 2000). "A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions". Proceedings of the National Academy of Sciences of the United States of America. 97 (13): 7382–7. doi:10.1073/pnas.110149297. PMC 16554. PMID 10852947.
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