Gyula Takátsy

Gyula Takátsy (1914–1980), was a Hungarian medical doctor, microbiologist and inventor.[1]

In 1938 he graduated from the University of Medicine in Pécs, Hungary;,[2] and joined the staff at the Institute of Public Health, where he worked until his early death in 1980.

An influenza epidemic in Hungary in the early 1950s led to a shortage of laboratory equipment, causing Takácsy to develop a number of new laboratory techniques and tools. These included small platinum spiral loops to replace pipettes, and microwell plates instead of test tubes. He introduced the idea of using calibrated spiral loops for multiple serial dilution in plastic microwell plates,[3] and coined the term 'micromethods', first published in Hungarian in 1952 and in English in 1955, for laboratory procedures carried out on a small scale. This allowed for the use of very small volumes of blood in virus research, and a considerable reduction in the use of costly serums and antigens.

The original spiral loop was similar to the platinum loop used in bacteriology - Takátsy improved it by forming multiple windings in the loop, somewhat like the filament of an incandescent light bulb. The narrow gaps between the windings drew a constant volume of fluid by capillary action. These were calibrated and arranged in arrays, enabling efficient dilution procedures. The reliability of this microtechnique led to its being adopted as the standard method for serological testing at the Communicable Disease Center.[4]

Takátsy carried out research on the variability of antigenic structure and the pathology and epidemiology of influenza virus mutations, as well as vaccine production and control, published in some 50 papers.

He was head of the Influenza Unit (WHO National Influenza Centre) which produced some 630 000 vaccine doses during the Hong Kong flu pandemic of 1968/1969.

Takátsy was the member of the Board of the Hungarian Society of Microbiology and received several awards, including the Manninger Rezső Medal.

In the course of time, microwell plates underwent many modifications. Vertical spectrophotometry readers using the Beer–Lambert law were developed and improved. Microwell plates and automated procedures have become standard in countless laboratories.[5]

References

  1. ↑ http://www.oek.hu/oek.web?nid=499&pid=1
  2. ↑ http://www.oek.hu/oek.web?nid=499&pid=1
  3. ↑ Takátsy, Gy: The Use of Spiral Loops in Serological and Virological Micromethods. Acta Microbiologica et Immunologica Hungarica, 3, pp 191-202 (1955)
  4. ↑ "APPLICATION OF A MICROTECHNIQUE TO THE AGGLUTINATION TEST FOR LEPTOSPIRAL ANTIBODIES". Appl Microbiol. 13: 81–5. January 1965. PMC 1058195. PMID 14264852.
  5. ↑ http://www.biotek.com/resources/docs/Necessity_is_the_mother_of_invention_GIT-Verlag.pdf
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