Genostar

Sequence assembly

• Mapping of an ensemble of sequences on a reference sequence

• between a reference sequence and contigs, between two sequences or between two sets of sequences
• finding of exact matches with minimum length using MUMmer
• detection of specific regions and SNPs
• creation of an assembled sequence relative to reference sequences

Genomic annotation

• Gene prediction: ab-initio gene prediction using a Hidden Markov model based method
• BlastX
• Automatic annotation transfer using BlastP

Proteic annotation

Metabolic Pathway Builder integrates several methods dedicated to proteic annotation:

• Pfam domain prediction using HMMER
• Several EMBOSS methods (antigenic, 2D structure prediction)

Expression Data Solution (EDS)

Genostar's Expression Data Solution (EDS) connects microarray data to genes, gene products and biochemical reactions, based on keywords and annotations. This software solution allows to:

• Assign expression values to the gene names and IDs
• Identify co-expressed genes and visually analyze the reactions and metabolic pathways in which they are involved
• Identify and perform analysis on co-regulated genes in terms of genomic localization, functional annotation and metabolism
• Colorize CDSs of interest in genomic maps according to their expression values and highlight the corresponding reactions in interactive metabolic KEGG maps
• Analyze the significance of functional data of a collection or sub-collection of CDSs (GO, KEGG and more): Fisher test
• Collect and visualize all functional data in exportable tables and maps