Galactoside acetyltransferase

GAT in complex with CoA and two molecules/active site of IPTG viewed perpendicular to the molecular threefold axis of the enzyme
Galactoside O-acetyltransferase
Identifiers
EC number 2.3.1.18
CAS number 9029-94-1
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / QuickGO
Thiogalactoside acetyltransferase
Identifiers
Organism Escherichia coli
Symbol lacA
Entrez 945674
RefSeq (Prot) NP_414876
UniProt P07464

Galactoside acetyltransferase (also known as Galactoside O-acetyltransferase, thiogalactoside transacetylase and GAT) is an enzyme that transfers an acetyl group from acetyl-CoA to galactosides, glucosides and lactosides. It is coded for by the lacA gene of the lac operon in E. coli.[1]

Reaction

It catalyzes the following reaction:

acetyl-CoA + beta-D-galactoside → CoA + 6-acetyl-beta-D-galactoside

The kinetics of the enzyme were delineated in 1995.[2]

Biological role

The enzyme's role in the classical E.coli lac operon remains unclear.[1][3] However, the enzyme's cellular role may be to detoxify non-metabolizable pyranosides by acetylating them and preventing their reentry into the cell.[4][5]

See also

References

  1. 1 2 Wang XG, Olsen LR, Roderick SL (April 2002). "Structure of the lac operon galactoside acetyltransferase". Structure. 10 (4): 581–8. doi:10.1016/S0969-2126(02)00741-4. PMID 11937062.
  2. ↑ Lewendon A, Ellis J, Shaw WV (November 1995). "Structural and mechanistic studies of galactoside acetyltransferase, the Escherichia coli LacA gene product". The Journal of Biological Chemistry. 270 (44): 26326–31. doi:10.1074/jbc.270.44.26326. PMID 7592843.
  3. ↑ Roderick SL (June 2005). "The lac operon galactoside acetyltransferase". Comptes Rendus Biologies. 328 (6): 568–75. doi:10.1016/j.crvi.2005.03.005. PMID 15950163.
  4. ↑ Wang XG, Olsen LR, Roderick SL (April 2002). "Structure of the lac operon galactoside acetyltransferase". Structure. 10 (4): 581–8. doi:10.1016/S0969-2126(02)00741-4. PMID 11937062.
  5. ↑ "Thiogalactoside transacetylase of the lactose operon as an enzyme for detoxification". 1976.


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