EFCBP2

NECAB2
Identifiers
AliasesNECAB2, EFCBP2, N-terminal EF-hand calcium binding protein 2, stip-2
External IDsMGI: 2152211 HomoloGene: 62200 GeneCards: NECAB2
Gene location (Human)
Chr.Chromosome 16 (human)[1]
Band16q23.3Start83,968,632 bp[1]
End84,002,776 bp[1]
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez

54550

117148

Ensembl

ENSG00000103154

ENSMUSG00000031837

UniProt

Q7Z6G3

Q91ZP9

RefSeq (mRNA)

NM_019065
NM_001329748
NM_001329749

NM_054095

RefSeq (protein)

NP_001316677
NP_001316678
NP_061938

NP_473436

Location (UCSC)Chr 16: 83.97 – 84 MbChr 8: 119.45 – 119.47 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

N-terminal EF-hand calcium-binding protein 2 is a protein that in humans is encoded by the NECAB2 gene.[5][6]

Model organisms

Model organisms have been used in the study of NECAB2 function. A conditional knockout mouse line, called Necab2tm1a(KOMP)Wtsi[11][12] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.[13][14][15]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[9][16] Twenty five tests were carried out on mutant mice but no significant abnormalities were observed.[9]

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000103154 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000031837 - Ensembl, May 2017
  3. ↑ "Human PubMed Reference:".
  4. ↑ "Mouse PubMed Reference:".
  5. ↑ Sugita S, Ho A, Sudhof TC (Jun 2002). "NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern". Neuroscience. 112 (1): 51–63. doi:10.1016/S0306-4522(02)00063-5. PMID 12044471.
  6. ↑ "Entrez Gene: EFCBP2 EF-hand calcium binding protein 2".
  7. ↑ "Salmonella infection data for Necab2". Wellcome Trust Sanger Institute.
  8. ↑ "Citrobacter infection data for Necab2". Wellcome Trust Sanger Institute.
  9. 1 2 3 Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x.
  10. ↑ Mouse Resources Portal, Wellcome Trust Sanger Institute.
  11. ↑ "International Knockout Mouse Consortium".
  12. ↑ "Mouse Genome Informatics".
  13. ↑ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
  14. ↑ Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
  15. ↑ Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
  16. ↑ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

  • Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
  • Yu W, Andersson B, Worley KC, et al. (1997). "Large-Scale Concatenation cDNA Sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
  • Bernier G, Vukovich W, Neidhardt L, et al. (2001). "Isolation and characterization of a downstream target of Pax6 in the mammalian retinal primordium". Development. 128 (20): 3987–94. PMID 11641222.
  • Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
  • Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
  • Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
  • Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
  • Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569.


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