(Formate-C-acetyltransferase)-activating enzyme
| [formate-C-acetyltransferase]-activating enzyme | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.97.1.4 | ||||||||
| CAS number | 206367-15-9 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a [formate-C-acetyltransferase]-activating enzyme (EC 1.97.1.4) is an enzyme that catalyzes the chemical reaction
- S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical
The 3 substrates of this enzyme are S-adenosyl-L-methionine, dihydroflavodoxin, and formate C-acetyltransferase-glycine, whereas its 4 products are 5'-deoxyadenosine, L-methionine, flavodoxin semiquinone, and formate C-acetyltransferase-glycin-2-yl radical.
This radical SAM enzyme belongs to the family of oxidoreductases. The systematic name of this enzyme class is [formate C-acetyltransferase]-glycine dihydroflavodoxin:S-adenosyl-L-methionine oxidoreductase (S-adenosyl-L-methionine cleaving). Other names in common use include PFL activase, PFL-glycine:S-adenosyl-L-methionine H transferase (flavodoxin-oxidizing, S-adenosyl-L-methionine-cleaving), formate acetyltransferase activating enzyme, formate acetyltransferase-glycine dihydroflavodoxin:S-adenosyl-L-methionine oxidoreductase (S-adenosyl-L-methionine cleaving).
References
- Frey M, Rothe M, Wagner AF, Knappe J (1994). "Adenosylmethionine-dependent synthesis of the glycyl radical in pyruvate formate-lyase by abstraction of the glycine C-2 pro-S hydrogen atom. Studies of [2H]glycine-substituted enzyme and peptides homologous to the glycine 734 site". J. Biol. Chem. 269 (17): 12432&ndash, 7. PMID 8175649.
- Wagner AF, Frey M, Neugebauer FA, Schafer W, Knappe J (1992). "The free radical in pyruvate formate-lyase is located on glycine-734". Proc. Natl. Acad. Sci. U.S.A. 89 (3): 996&ndash, 1000. doi:10.1073/pnas.89.3.996. PMC 48372. PMID 1310545.
- Frey PA (2001). "Radical mechanisms of enzymatic catalysis". Annu. Rev. Biochem. 70: 121&ndash, 48. doi:10.1146/annurev.biochem.70.1.121. PMID 11395404.