Venomous snake

Venom toxicities are compared by looking at the median lethal dose (usually using rodents as test animals and termed the murine LD₅₀), which is the dose of venom per unit body mass that kills half of the test animals that receive it. The result obtained depends on which of the four delivery sites is used for the injection: subcutis (SC), vein (IV), muscle (IM or IC), or peritoneum (IP). Smaller murine LD₅₀ values indicate venoms that are more toxic, and there have been numerous studies on snake venom with a variability of potency estimates.[4] SC LD₅₀ is considered the most applicable to actual bites as only vipers with large fangs (such as large specimens from the genera Bitis, Bothrops, Crotalus, or Daboia) are capable of a truly intramuscular bite, snakebites rarely cause IV envenomation, and IP envenomation is even rarer. Measurements of LD₅₀ using dry venom mixed with 0.1% bovine serum albumin in saline are more consistent than the results obtained using saline alone. As an example, the venom of the eastern brown snake has a murine LD₅₀ (SC) of 41 μg/kg when measured in 0.1% bovine serum albumin in saline; when saline alone is used, the value is 53 μg/kg.[5]

Belcher's sea snake (Hydrophis belcheri), which sometimes is mistakenly called the hook-nosed Sea Snake (Enhydrina schistosa), has been erroneously popularized as the most venomous snake in the world, due to the first edition of Ernst and Zug's book, Snakes in Question: The Smithsonian Answer Book, published in 1996. Prominent venom expert Associate Professor Bryan Grieg Fry has clarified the error: "The hook nosed myth was due to a fundamental error in a book called Snakes in Question. In there, all the toxicity testing results were lumped in together, regardless of the mode of testing (e.g., subcutaneous vs. intramuscular vs. intravenous vs. intraperitoneal). As the mode can influence the relative number, venoms can only be compared within a mode. Otherwise, it's apples and rocks."[6] Belcher's sea snake's actual LD₅₀ (IM) is 0.24 mg/kg[7] and 0.155 mg/kg.[8] Studies on mice[9][10][11] and human cardiac cell culture[6][12][13] show that venom of the inland taipan, drop by drop, is the most toxic among all snakes.

Common krait (Bungarus caeruleus)

Russell's viper (Daboia russelii)

Indian cobra (Naja naja)

Saw-scaled viper (Echis carinatus)

The Big Four snakes responsible for most fatal bites on the Indian Subcontinent

The toxicity of snake venom [based on laboratory tests conducted on mice] is sometimes used to gauge the extent of danger to humans, but this is not enough. Many venomous snakes are specialized predators whose venom may be adapted specifically to incapacitate their preferred prey.[15] A number of other factors are also critical in determining the potential hazard of any given venomous snake to humans, including their distribution and behavior.[16][17] For example, while the inland taipan is regarded as the world's most venomous snake based on LD₅₀ tests on mice, it is a shy species and rarely strikes, and has not caused any known human fatalities. On the other hand, India's Big Four (Indian Cobra, common Krait, Russell's viper, and saw-scaled viper), while less venomous than the inland taipan, are found in closer proximity to human settlements and are more confrontational, thus leading to more deaths from snakebite. In addition, some species, such as the black mamba and coastal taipan, occasionally show some aggression, generally when alarmed or in self-defence, and then may deliver fatal doses of venom, resulting in high human mortality rates.[18][19]