Toluidine blue stain

Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components.[1]

Toluidine blue stain in a vasculitic peripheral neuropathy.

It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images. It is especially useful today for staining chromosomes in plant or animal tissues, as a replacement for Aceto-orcein stain.

Results

The results depend on the studied organs:[2]

- Mastocytes in purple

- Cartilage in purple

- Mucins in purple/red

- Nuclei in blue

It is used in forensic examination,[3] renal pathology[4] and neuropathology.

See also

References
  1. Sridharan, G; Shankar, AA (2012). "Toluidine blue: A review of its chemistry and clinical utility". J Oral Maxillofac Pathol. 16: 251–5. doi:10.4103/0973-029X.99081. PMC 3424943. PMID 22923899.
  2. "Toluidine blue". Histalim. Archived from the original on 2018-07-01. Retrieved 2020-03-15.
  3. Olshaker, Jackson and Smock (2001). Forensic Emergency Medicine. Philadelphia: Lippincott, Williams and Williams. pp. 94–97. ISBN 0781731445.
  4. Nicholas, SB.; Basgen, JM.; Sinha, S. (2011). "Using stereologic techniques for podocyte counting in the mouse: shifting the paradigm". Am J Nephrol. 33 Suppl 1: 1–7. doi:10.1159/000327564. PMC 3121548. PMID 21659728.
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